Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, China.
Department of General Surgery, Qilu Hospital of Shandong University, Jinan, 250012, China.
Curr Med Sci. 2022 Jun;42(3):538-547. doi: 10.1007/s11596-022-2597-1. Epub 2022 Jun 8.
Increasing evidence has indicated that there is a correlation between Fusobacterium nucleatum (F. nucleatum) abundance and poor prognosis of colorectal cancer (CRC). Furthermore, tumor metastasis plays a decisive role in the prognosis of CRC patients. Therefore, it was hypothesized that the abundance of F. nucleatum in CRC tissues affects the tumor metastasis.
In the present study, F. nucleatum DNA obtained from 141 resected CRC samples was quantified by qPCR to determine whether there were differences in F. nucleatum abundance between groups with and without CRC metastasis.
The results revealed that F. nucleatum was more abundant in CRC patients with metastasis, and CRC tissues enriched with F. nucleatum had a higher risk of lymph node metastasis and distant metastasis. The receiver operating characteristic curve indicated that F. nucleatum in CRC tissues could be used as an indicator for CRC metastasis, to some extent. Furthermore, the in vitro experiments (electron microscopy, and migration and invasion trials) revealed that F. nucleatum was a highly invasive bacterial strain, and could significantly enhance the invasion and migration capacity of SW480 and SW620 cells. In addition, a meta-analysis comprehensively indicated a slight correlation between F. nucleatum abundance and advanced CRC stage (RR=1.17, 95% CI: 1.00-1.37, P=0.04, random effect).
There is a correlation between F. nucleatum abundance and CRC metastasis, and F. nucleatum may serve as a metastasis biomarker for CRC patients.
越来越多的证据表明，具核梭杆菌（F. nucleatum）丰度与结直肠癌（CRC）的预后不良之间存在相关性。此外，肿瘤转移在 CRC 患者的预后中起决定性作用。因此，推测 CRC 组织中 F. nucleatum 的丰度会影响肿瘤转移。
本研究通过 qPCR 定量检测 141 例切除的 CRC 样本中的 F. nucleatum DNA，以确定有无 CRC 转移的组之间 F. nucleatum 丰度是否存在差异。
结果表明，转移性 CRC 患者中 F. nucleatum 更为丰富，富含 F. nucleatum 的 CRC 组织发生淋巴结转移和远处转移的风险更高。受试者工作特征曲线表明，CRC 组织中的 F. nucleatum 可在一定程度上作为 CRC 转移的指标。此外，体外实验（电子显微镜、迁移和侵袭试验）表明，F. nucleatum 是一种侵袭性很强的细菌株，可显著增强 SW480 和 SW620 细胞的侵袭和迁移能力。此外，荟萃分析综合表明，F. nucleatum 丰度与晚期 CRC 分期之间存在轻微相关性（RR=1.17，95%CI：1.00-1.37，P=0.04，随机效应）。
F. nucleatum 丰度与 CRC 转移之间存在相关性，F. nucleatum 可能成为 CRC 患者的转移生物标志物。
