Whitehead R H, Jones J K, Gabriel A, Lukies R E
Cancer Res. 1987 May 15;47(10):2683-9.
A new colon carcinoma cell line (LIM1863) has been characterized. This cell line is unique in that the culture consists of organoids which are morphologically and functionally organized. Histological studies of the organoids show that the cells are arranged around a central lumen and the nuclei are polarized to the periphery. Two major morphological types are present: a columnar cell with a polarized, structurally normal brush border and goblet cells. The cells are also functionally mature and express the brush border enzymes aminopeptidase N, dipeptidyl peptidase IV, alkaline phosphatase, and sucrase-isomaltase. These enzymes are localized to the luminal membrane and the apical cytoplasm (of some cells). The goblet cells contain mucus and this mucus is secreted into the lumen. This functional differentiation suggests that the organoids contain precursor cells capable of differentiating along both the columnar and goblet cell pathways. At present no endocrine cells have been detected by morphological or histochemical analysis. The organoids have been in continuous culture with regular passaging for 21 months and also grow and differentiate normally in serum-free medium.
一种新的结肠癌细胞系（LIM1863）已被鉴定。该细胞系的独特之处在于其培养物由形态和功能上有组织的类器官组成。对类器官的组织学研究表明，细胞围绕中央管腔排列，细胞核向周边极化。存在两种主要的形态类型：具有极化、结构正常的刷状缘的柱状细胞和杯状细胞。这些细胞在功能上也已成熟，表达刷状缘酶氨肽酶N、二肽基肽酶IV、碱性磷酸酶和蔗糖酶-异麦芽糖酶。这些酶定位于管腔膜和（某些细胞的）顶端细胞质。杯状细胞含有黏液，且这种黏液分泌到管腔中。这种功能分化表明类器官含有能够沿柱状细胞和杯状细胞途径分化的前体细胞。目前，通过形态学或组织化学分析未检测到内分泌细胞。这些类器官已连续传代培养21个月，并且在无血清培养基中也能正常生长和分化。
