Whitehead R H, van Eeden P, Lukeis R E
Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Royal Melbourne Hospital, Victoria, Australia.
Int J Cancer. 1991 Jul 9;48(5):693-6. doi: 10.1002/ijc.2910480511.
We describe a new epithelial cell line (LIM 2463) derived from tubulovillous adenoma of the rectum. The cells grow as organoids and secrete large amounts of mucus. The cells are polarized, with a microvillar brush border, and express dipeptidyl peptidase IV in a polarized manner. No staining was seen with 3 other antibodies directed against other brush-border hydrolases or disaccharidases. Focal polarized staining was obtained with 2 antibodies directed against other brush-border-associated peptides. The cells are aneuploid with a distinctive karyotype (48, XX, +9, +13). All attempts to clone the cells in semi-solid agar or to grow them as xenografts in nude athymic mice have failed.
我们描述了一种新的上皮细胞系（LIM 2463），它源自直肠管状绒毛状腺瘤。这些细胞以类器官形式生长并分泌大量黏液。细胞呈极化状态，具有微绒毛刷状缘，并以极化方式表达二肽基肽酶IV。针对其他刷状缘水解酶或双糖酶的另外3种抗体未显示染色。针对其他刷状缘相关肽的2种抗体获得了局灶性极化染色。这些细胞为非整倍体，具有独特的核型（48, XX, +9, +13）。在半固体琼脂中克隆这些细胞或在无胸腺裸鼠中作为异种移植物培养它们的所有尝试均告失败。
