College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, China.
Anal Chem. 2022 Jun 21;94(24):8693-8703. doi: 10.1021/acs.analchem.2c01008. Epub 2022 Jun 9.
The color-enzyme lateral flow immunoassay (LFIA) has attracted widespread attention to expand the detection range and improve sensitivity via amplifying the color signal after catalyzing the substrate. As a kind of layered transition-metal dichalcogenide (TMD), the vanadium disulfide nanosheet (VSNS) possesses superior peroxidase-like catalytic activity. Here, a VSNS was applied as an enzyme label in the LFIA to detect 17β-estradiol (E2). Compared to natural horseradish peroxidase, the VSNS expresses a more prominent enzyme catalytic performance, stability, and adsorption ability. Under optimal conditions, the calculated limit of detection (cLOD) of the VSNS-based LFIA is 0.065 ng mL for E2, which is sixfold lower than that of the optimized colloidal nanoparticle-based LFIA (cLOD = 0.406 ng mL). Besides, the detection linear range of the VSNS-based LFIA can be widened by 1.5 times after the catalytic reaction. Moreover, the VSNS-based LFIA exhibits excellent practicability in real sample detection. Simultaneously, this study helps open up the application of the VSNS in the trace analysis of LFIAs, which can broaden TMDs' scope of application and better show their properties of color enzymes.
基于纳米酶的比色免疫层析法检测 17β-雌二醇
颜色-酶侧向流动免疫分析（LFIA）通过在催化底物后放大颜色信号，从而吸引了广泛的关注，以扩大检测范围并提高灵敏度。作为一种层状过渡金属二硫属化物（TMD），二硫化钒纳米片（VSNS）具有优异的过氧化物酶样催化活性。在这里，将 VSNS 用作 LFIA 中的酶标记物来检测 17β-雌二醇（E2）。与天然辣根过氧化物酶相比，VSNS 表现出更突出的酶催化性能、稳定性和吸附能力。在最佳条件下，基于 VSNS 的 LFIA 的计算检测限（cLOD）为 0.065ng/mL 的 E2，比优化的胶体纳米颗粒基 LFIA（cLOD=0.406ng/mL）低 6 倍。此外，催化反应后，基于 VSNS 的 LFIA 的检测线性范围可以拓宽 1.5 倍。此外，基于 VSNS 的 LFIA 在实际样品检测中表现出优异的实用性。同时，本研究有助于开拓 VSNS 在 LFIA 痕量分析中的应用，拓宽 TMDs 的应用范围，更好地展示其颜色酶的性能。
