School of Materials and Chemical Engineering, Xi'an Technological University, Xi'an 710021, China.
Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, China.
Biosensors (Basel). 2022 Nov 7;12(11):977. doi: 10.3390/bios12110977.
caused by viral or bacterial infection is a major threat to human health globally. Blood C-reactive protein (CRP) has been proven to be a sensitive indicator for the occurrence and development of inflammation. Furthermore, a tiny change of blood CRP concentration may portend chronic diseases; therefore, high-sensitivity CRP (hs-CRP) detection in a quantitative, rapid, user-friendly, and low-cost manner is highly demanded. In this paper, we developed a europium-chelate polystyrene microsphere (EuPSM)-based lateral flow immunoassay (LFIA) integrating with a benchtop fluorescence analyzer for hs-CRP detection. The optimization of the EuPSM-based LFIA was implemented through adjusting the antibody density on EuPSM from 100% to 60% of the saturated density. Finally, the limit of detection of 0.76 pg/mL and detection range of 0.025-250 ng/mL were obtained. Moreover, the clinical application capability of the proposed platform was validated through detecting CRP in clinical serum samples, showing high consistency with the results obtained from the clinical standard method. Hence, the proposed EuPSM-based LFIA has been verified to be well suitable for hs-CRP detection, while also showing great applicability for sensitively and rapidly detecting other biomarkers.
由病毒或细菌感染引起的炎症是全球范围内对人类健康的主要威胁。血液 C 反应蛋白（CRP）已被证明是炎症发生和发展的敏感指标。此外，血液 CRP 浓度的微小变化可能预示着慢性疾病的发生；因此，人们迫切需要以定量、快速、用户友好和低成本的方式进行高灵敏度 CRP（hs-CRP）检测。在本文中，我们开发了一种基于铕螯合物聚苯乙烯微球（EuPSM）的侧向流动免疫分析（LFIA），并结合台式荧光分析仪用于 hs-CRP 检测。通过将 EuPSM 上的抗体密度从饱和密度的 100%调整到 60%，实现了基于 EuPSM 的 LFIA 的优化。最终，获得了 0.76 pg/mL 的检测限和 0.025-250 ng/mL 的检测范围。此外，通过检测临床血清样本中的 CRP，验证了该平台的临床应用能力，与临床标准方法的结果高度一致。因此，所提出的基于 EuPSM 的 LFIA 已被证明非常适合 hs-CRP 检测，同时也非常适用于灵敏快速地检测其他生物标志物。
