Abid Sanae, Maciuk Alexander, Fishmeister Rodolphe, Leblais Véronique, Legssyer Abdekhaleq, Mekhfi Hassane, Ziyyat Abderrahim, Aziz Mohamed, Lekchiri Abdenbi, Bnouham Mohamed
Laboratory of Bio-resources, Biotechnologies, Ethnopharmacology and health, Department of Biology. Faculty of Sciences, University Mohamed Ist, Boulevard Mohamed IV, BP: 717, Oujda 60 000, Morocco.
Laboratory of Pharmacognosy, Faculty of Pharmacy, University Paris-Saclay, 5 Rue Jean Baptiste Clément, 92290 Châtenay-Malabry, France.
J Chromatogr Sci. 2022 Dec 29;61(1):66-73. doi: 10.1093/chromsci/bmac040.
A rapid, easy and simple method for the isolation and purification of α-glucosidase inhibitors of the ethyl acetate extract of Thymelaea hirsuta (EaTh) by a combination of thin layer chromatography (TLC) and enzymatic test has been developed. EaTh was demonstrated previously a potent α-glucosidase inhibitory effect. In this study, we developed a simple TLC-enzymatic test (TLC/EZ) combination to isolate α-glucosidase inhibitors present in EaTh.EaTh was extracted by Soxhlet from Thymelaea hirsuta (T. hirsuta). The EaTh was separated on a silica gel column and then on a TLC plate. After TLC separation, the TLC/EZ combination method was applied. α-glucosidase inhibitors were detected directly in the TLC plate using the glucose oxidase peroxidase method (GOD-POD). A good detection of active compounds was obtained in the TLC favoring the TLC/EZ method. Active compounds were then characterized using high performance liquid chromatography-mass spectrometry (HPLC-MS) analysis. The main α-glucosidase inhibitors present in EaTh have a molecular ion [M + H]+ at m/z = 543. This proposed method is suitable for a reliable isolation and purification of α-glucosidase inhibitors present in EaTh. It could be proposed as an interesting alternative of the classical method for the isolation and purification of α-glucosidase inhibitors in plant extracts.
已经开发出一种通过薄层色谱（TLC）和酶促试验相结合的快速、简便的方法，用于分离和纯化多毛瑞香乙酸乙酯提取物（EaTh）中的α-葡萄糖苷酶抑制剂。先前已证明EaTh具有强大的α-葡萄糖苷酶抑制作用。在本研究中，我们开发了一种简单的TLC-酶促试验（TLC/EZ）组合方法，以分离EaTh中存在的α-葡萄糖苷酶抑制剂。EaTh通过索氏提取法从多毛瑞香（T. hirsuta）中提取。EaTh先在硅胶柱上分离，然后在TLC板上分离。TLC分离后，应用TLC/EZ组合方法。使用葡萄糖氧化酶过氧化物酶法（GOD-POD）直接在TLC板上检测α-葡萄糖苷酶抑制剂。在TLC中对活性化合物进行了良好的检测，这有利于TLC/EZ方法。然后使用高效液相色谱-质谱（HPLC-MS）分析对活性化合物进行表征。EaTh中存在的主要α-葡萄糖苷酶抑制剂的分子离子[M + H]+的质荷比为m/z = 543。该方法适用于可靠地分离和纯化EaTh中存在的α-葡萄糖苷酶抑制剂。它可以作为植物提取物中α-葡萄糖苷酶抑制剂分离和纯化经典方法的一种有趣替代方法。
