多毛瑞香提取物对α-葡萄糖苷酶及葡萄糖肠道吸收的抑制作用
Inhibition of α-glucosidase and glucose intestinal absorption by Thymelaea hirsuta fractions.
作者信息
Abid Sanae, Lekchiri Abdenbi, Mekhfi Hassane, Ziyyat Abderrahim, Legssyer Abdekhaleq, Aziz Mohamed, Bnouham Mohamed
机构信息
Laboratory of Physiology and Ethnopharmacology, Department of Biology, Faculty of Sciences, University Mohamed Ist, Oujda, Morocco.
出版信息
J Diabetes. 2014 Jul;6(4):351-9. doi: 10.1111/1753-0407.12106. Epub 2014 Jan 9.
BACKGROUND
Thymelaea hirsuta (L.) Endl. (Thymelaeaceae) is a medicinal plant used in Morocco to treat diabetes. In previous studies T. hirsuta has shown a potent antihyperglycemic effect. Our aim was to study the effect of the plant on α-glucosidase inhibition and intestinal glucose absorption.
METHODS
Five fractions of T. hirsuta were tested, in vitro, in vivo and, in situ, to elucidate the inhibition of α-glucosidase and intestinal glucose uptake.
RESULTS
The fractions induced, in vitro, a significant inhibition of α-glucosidase. The ethyl acetate fraction (EATh) had high activity and its inhibition mode was non-competitive. The EATh at 50 and 100 mg/kg doses, decreased significantly, in vivo, the postprandial hyperglycemia after sucrose loading in normal and diabetic mice. Moreover, 50 mg/kg of EATh significantly decreased intestinal glucose uptake, in situ, in rats.
CONCLUSION
The antihyperglycemic effect of T. hirsuta can be explained, in part, by the inhibition of intestinal α-glucosidase and intestinal glucose absorption.
背景
多毛瑞香（Thymelaea hirsuta (L.) Endl.，瑞香科）是摩洛哥用于治疗糖尿病的一种药用植物。在先前的研究中，多毛瑞香已显示出强大的降血糖作用。我们的目的是研究该植物对α - 葡萄糖苷酶抑制和肠道葡萄糖吸收的影响。
方法
对多毛瑞香的五个部位进行了体外、体内和原位测试，以阐明其对α - 葡萄糖苷酶的抑制作用和肠道葡萄糖摄取情况。
结果
这些部位在体外对α - 葡萄糖苷酶有显著抑制作用。乙酸乙酯部位（EATh）活性较高，其抑制模式为非竞争性。50和100mg/kg剂量的EATh在体内可显著降低正常和糖尿病小鼠蔗糖负荷后的餐后高血糖。此外，50mg/kg的EATh可显著降低大鼠原位肠道葡萄糖摄取。
结论
多毛瑞香的降血糖作用部分可通过抑制肠道α - 葡萄糖苷酶和肠道葡萄糖吸收来解释。
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