Abid Sanae, Lekchiri Abdenbi, Mekhfi Hassane, Ziyyat Abderrahim, Legssyer Abdekhaleq, Aziz Mohamed, Bnouham Mohamed
Laboratory of Physiology and Ethnopharmacology, Department of Biology, Faculty of Sciences, University Mohamed Ist, Oujda, Morocco.
J Diabetes. 2014 Jul;6(4):351-9. doi: 10.1111/1753-0407.12106. Epub 2014 Jan 9.
Thymelaea hirsuta (L.) Endl. (Thymelaeaceae) is a medicinal plant used in Morocco to treat diabetes. In previous studies T. hirsuta has shown a potent antihyperglycemic effect. Our aim was to study the effect of the plant on α-glucosidase inhibition and intestinal glucose absorption.
Five fractions of T. hirsuta were tested, in vitro, in vivo and, in situ, to elucidate the inhibition of α-glucosidase and intestinal glucose uptake.
The fractions induced, in vitro, a significant inhibition of α-glucosidase. The ethyl acetate fraction (EATh) had high activity and its inhibition mode was non-competitive. The EATh at 50 and 100 mg/kg doses, decreased significantly, in vivo, the postprandial hyperglycemia after sucrose loading in normal and diabetic mice. Moreover, 50 mg/kg of EATh significantly decreased intestinal glucose uptake, in situ, in rats.
The antihyperglycemic effect of T. hirsuta can be explained, in part, by the inhibition of intestinal α-glucosidase and intestinal glucose absorption.
多毛瑞香（Thymelaea hirsuta (L.) Endl.，瑞香科）是摩洛哥用于治疗糖尿病的一种药用植物。在先前的研究中，多毛瑞香已显示出强大的降血糖作用。我们的目的是研究该植物对α - 葡萄糖苷酶抑制和肠道葡萄糖吸收的影响。
对多毛瑞香的五个部位进行了体外、体内和原位测试，以阐明其对α - 葡萄糖苷酶的抑制作用和肠道葡萄糖摄取情况。
这些部位在体外对α - 葡萄糖苷酶有显著抑制作用。乙酸乙酯部位（EATh）活性较高，其抑制模式为非竞争性。50和100mg/kg剂量的EATh在体内可显著降低正常和糖尿病小鼠蔗糖负荷后的餐后高血糖。此外，50mg/kg的EATh可显著降低大鼠原位肠道葡萄糖摄取。
多毛瑞香的降血糖作用部分可通过抑制肠道α - 葡萄糖苷酶和肠道葡萄糖吸收来解释。
