Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
Department of Applied Animal Science, College of Animal Life Science, Kangwon National University, Chuncheon 24341, Republic of Korea.
Poult Sci. 2022 Jul;101(7):101953. doi: 10.1016/j.psj.2022.101953. Epub 2022 May 6.
The objective of the present study was to compare the breast meat quality and metabolomic characteristics from broilers that were raised in conventional (conventional farm reared-broilers; CB, n = 20) and legally approved animal welfare farms (welfare farm reared-broilers; WB, n = 20) in aerobic cold storage (1, 3, 5, and 7 d). Compared to CB chickens, the WB chickens had a larger floor size as well as lower stocking density, atmospheric ammonia, and nipple-shared chicken counts. The results demonstrated significantly higher pH, L- and b-value, and lower shear force in CB compared to WB during cold storage. Using H NMR analysis, 25 compounds were identified in the chicken breast meat. Partial least square-discriminant analysis (PLS-DA) was performed based on the identified metabolites. The content of 15 metabolites (1 di-peptide, 9 free amino acids, 2 glycolytic potential-related products, 2 nucleotide-related products, and 1 organic acid) was significantly different due to the rearing environment (CB vs. WB). Among them, all free amino acids were higher in CB than in WB. Six free amino acids (glycine, isoleucine, leucine, phenylalanine, valine, and β-alanine) had variable importance in projection (VIP) score >1, regardless of the number of cold storage days. Therefore, these compounds in the breast meat may be used as potential markers to determine the rearing environment of broilers. Also, this result might be an indication of stress-related meat quality changes in broilers.
本研究的目的是比较在常规（常规农场饲养肉鸡；CB，n=20）和合法批准的动物福利农场（福利农场饲养肉鸡；WB，n=20）中饲养的肉鸡在有氧冷藏（1、3、5 和 7 天）条件下的胸肉质量和代谢组学特征。与 CB 鸡相比，WB 鸡的地面面积更大，饲养密度、大气氨和共用乳头的鸡数量更低。结果表明，在冷藏过程中，CB 鸡肉的 pH 值、L-和 b-值显著高于 WB，而剪切力则较低。使用 H NMR 分析，在鸡胸肉中鉴定出 25 种化合物。基于鉴定出的代谢物进行偏最小二乘判别分析（PLS-DA）。由于饲养环境（CB 与 WB）的不同，有 15 种代谢物（1 种二肽、9 种游离氨基酸、2 种糖酵解潜能相关产物、2 种核苷酸相关产物和 1 种有机酸）的含量存在显著差异。其中，所有游离氨基酸在 CB 中的含量均高于 WB。6 种游离氨基酸（甘氨酸、异亮氨酸、亮氨酸、苯丙氨酸、缬氨酸和β-丙氨酸）的变量重要性投影（VIP）得分>1，与冷藏天数无关。因此，这些胸肉中的化合物可能被用作确定肉鸡饲养环境的潜在标志物。此外，这一结果可能表明肉鸡的应激相关肉质变化。
