College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Institution of Traditional Chinese Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Harbin 150030, PR China.
Poult Sci. 2022 Jul;101(7):101938. doi: 10.1016/j.psj.2022.101938. Epub 2022 Apr 30.
Preovulatory follicles need a network of blood vessels to growth and maturation in hens (Gallus gallus). Angelica sinensis (Oliv.) (AS), a traditional Chinese herb, displays a novel pro-angiogenic activity. The molecular mechanisms underlying AS promoting preovulatory follicles angiogenesis are poorly understand. Several recent studies investigated the expression of vascular endothelial growth factor A (VEGF-A) in angiogenesis. In order to explore the promotion effect of AS extract on angiogenesis of chicken preovulatory follicles, we studied the effect of AS extract on follicle microvascular endothelial-like cells of chicken (FMEC) and granulosa cells (GC). The current study indicated that AS extract could promote the proliferation of FMECs and GCs. The assays of wounding healing, transwell invasion and tube formation showed that AS extract could enhance the invasion and migration ability of FMECs in vitro. The results of western blot and RT-PCR showed that AS extract promoted the phosphorylation of vascular endothelial growth factor receptor 2 (VEGFR2) in FMECs by activating the PI3K/AKT signaling pathway. The AS extract activated PI3K/AKT signaling pathway and up-regulated the expressions of hypoxia-inducible factor 1-α (HIF1-α) and VEGF-A in GCs. In addition, treatment of FMECs and GCs with LY294002 (a PI3K inhibitor) significantly down-regulated the phosphorylation of VEGFR2, VEGF-A, and HIF1-α. The mRNA expression levels of PI3K, AKT, VEGF-A, VEGFR2, and HIF1-α were consistent with protein expression levels. In conclusion, our research showed that AS extract can promote the follicle angiogenesis in hens in vitro, providing a basis for application of the traditional Chinese herb AS in poultry production.
在母鸡（Gallus gallus）中，排卵前卵泡需要血管网络来生长和成熟。当归（Angelica sinensis （Oliv.））是一种传统的中草药，具有新颖的促血管生成活性。当归促进排卵前卵泡血管生成的分子机制尚未完全了解。最近的几项研究调查了血管内皮生长因子 A（VEGF-A）在血管生成中的表达。为了探讨当归提取物对鸡排卵前卵泡血管生成的促进作用，我们研究了当归提取物对鸡卵泡微血管内皮样细胞（FMEC）和颗粒细胞（GC）的影响。本研究表明，当归提取物可促进 FMEC 和 GC 的增殖。划痕愈合、Transwell 侵袭和管形成测定表明，当归提取物可增强 FMEC 的体外侵袭和迁移能力。Western blot 和 RT-PCR 的结果表明，当归提取物通过激活 PI3K/AKT 信号通路促进 FMEC 中血管内皮生长因子受体 2（VEGFR2）的磷酸化。当归提取物激活 PI3K/AKT 信号通路并上调 GC 中缺氧诱导因子 1-α（HIF1-α）和 VEGF-A 的表达。此外，用 LY294002（PI3K 抑制剂）处理 FMEC 和 GC 可显著下调 VEGFR2、VEGF-A 和 HIF1-α的磷酸化。PI3K、AKT、VEGF-A、VEGFR2 和 HIF1-α 的 mRNA 表达水平与蛋白表达水平一致。总之，我们的研究表明，当归提取物可在体外促进母鸡卵泡血管生成，为传统中草药当归在禽类生产中的应用提供了依据。
