Laboratory of Aquaculture Nutrition and Environmental Health (LANEH), School of Life Sciences, East China Normal University, Shanghai 200241, China.
The Key Laboratory of Mariculture, Ministry of Education, The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture, Ocean University of China, Qingdao 266003, China.
Food Chem. 2022 Nov 1;393:133392. doi: 10.1016/j.foodchem.2022.133392. Epub 2022 Jun 3.
Flesh quality is influenced by diet components, but the underlying mechanism remains unclear. This study aimed to investigate the effect of replacing soybean meal (SBM) protein with cottonseed protein concentrate (CPC) at different levels (0%, CK; 15%, CPC15; 30%, CPC30 and 45%, CPC45) on the flesh quality of Nile tilapia. The results indicated that different protein sources influenced muscle amino acid composition instead of fatty acid composition. Lower muscle lipid content was found in CPC45, which in turn significantly altered the muscle texture. The hepatic lipid metabolism-related genes were detected and we found that CPC45 significantly suppressed the lipogenesis and promoted lipolysis. Higher content of microbiota-derived butyrate was found in the intestinal content of CPC45 and butyrate could decrease the lipid accumulation in vitro. Replacing SBM with CPC increased the intestinal butyrate to suppress the lipogenesis in the liver which may account for the increased muscle hardness.
肉质受饮食成分的影响，但其中的作用机制尚不清楚。本研究旨在探讨用棉籽浓缩蛋白（CPC）替代不同水平的大豆蛋白（SBM）（0%，CK；15%，CPC15；30%，CPC30；45%，CPC45）对尼罗罗非鱼肉质的影响。结果表明，不同的蛋白质来源影响肌肉的氨基酸组成，而不是脂肪酸组成。CPC45 组肌肉中的脂质含量较低，这反过来又显著改变了肌肉的质地。检测了与肝脏脂质代谢相关的基因，发现 CPC45 显著抑制了脂肪生成，促进了脂肪分解。CPC45 组肠道内容物中存在更多的微生物衍生的丁酸，而丁酸在体外可以减少脂质积累。用 CPC 替代 SBM 增加了肠道中的丁酸，从而抑制了肝脏中的脂肪生成，这可能是肌肉硬度增加的原因。
