基于金纳米簇酶样活性调控的碘离子和汞离子比色检测。

Colorimetric detections of iodide and mercuric ions based on a regulation of an Enzyme-Like activity from gold nanoclusters.

机构信息

The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2022 Oct 15;279:121450. doi: 10.1016/j.saa.2022.121450. Epub 2022 May 31.

DOI:10.1016/j.saa.2022.121450

PMID:35679739

Abstract

A simple colorimetric method was developed for sensitive and selective detections of I and Hg. Histidine stabilized gold nanoclusters (His-AuNCs) were synthesized and catalyzed the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) to a blue product. As a strong ligand toward gold, iodide (I) attached to the surface of the His-AuNCs and significantly enhanced the oxidase-like activity of the His-AuNCs. Based on this enhancement, a sensitive colorimetric response toward I was obtained. Furthermore, the strong interaction between Hg and I was adopted for an indirect Hg detection. Under the optimal conditions, the platform presented high selectivity for the determinations of I and Hg in the ranges 0.02-1 µM and 0.05-0.8 µM, with detection limits as 3.3 nM and 8 nM respectively. This colorimetric assay was successfully applied for analysis of real samples.

摘要

开发了一种简单的比色法，用于灵敏和选择性检测 I 和 Hg。合成了组氨酸稳定的金纳米簇（His-AuNCs），并催化无色 3,3',5,5'-四甲基联苯胺（TMB）氧化为蓝色产物。作为对金的强配体，碘化物（I）附着在 His-AuNCs 的表面上，显著增强了 His-AuNCs 的氧化酶样活性。基于这种增强，获得了对 I 的敏感比色响应。此外，采用 Hg 和 I 之间的强相互作用进行间接 Hg 检测。在最佳条件下，该平台对 I 和 Hg 的测定具有高选择性，范围分别为 0.02-1 µM 和 0.05-0.8 µM，检测限分别为 3.3 nM 和 8 nM。该比色法分析成功应用于实际样品分析。