Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, Institute of Ecology, School of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China.
Key Laboratory of Humid Subtropical Eco-geographical Process, Ministry of Education, Fujian Normal University, Fuzhou 350007, China.
Sci Total Environ. 2022 Sep 10;838(Pt 3):156534. doi: 10.1016/j.scitotenv.2022.156534. Epub 2022 Jun 6.
Nitrite-dependent anaerobic methane oxidation (n-damo), catalyzed by bacteria closely related to Candidatus Methylomirabilis oxyfera, links the global carbon and nitrogen cycles. Currently, the contribution of n-damo in controlling methane emissions and nitrogen removal, and the key regulatory factors of this process in Chinese paddy fields are poorly known. Here, soil samples from 20 paddy fields located in different climate zones across China were collected to examine the n-damo activity and bacterial communities. The n-damo activity and bacterial abundance varied from 1.05 to 5.97 nmol CH g (dry soil) d and 2.59 × 10 to 2.50 × 10 copies g dry soil, respectively. Based on the n-damo activity, it was estimated that approximately 0.91 Tg CH and 2.17 Tg N could be consumed annually via n-damo in Chinese paddy soils. The spatial variations in n-damo activity and community structure of n-damo bacteria were significantly (p < 0.05) affected by the soil ammonium content, labile organic carbon content and pH. Furthermore, significant differences in n-damo activity, bacterial abundance and community composition were observed among different climate zones. The n-damo activity was found to be positively correlated with the mean annual air temperature. Taken together, our results demonstrated the potential importance of n-damo in both methane consumption and nitrogen removal in Chinese paddy soils, and this process was regulated by local soil and climatic factors.
亚硝酸盐依赖型厌氧甲烷氧化（n-damo）由与 Candidatus Methylomirabilis oxyfera 密切相关的细菌催化，将全球碳氮循环联系起来。目前，n-damo 在控制甲烷排放和去除氮素中的贡献以及该过程在中国稻田中的关键调控因子知之甚少。本研究从中国不同气候带的 20 个稻田采集土壤样本，以检测 n-damo 活性和细菌群落。n-damo 活性和细菌丰度分别为 1.05 至 5.97 nmol CH g（干土）d 和 2.59×10 至 2.50×10 拷贝 g 干土。基于 n-damo 活性，估计每年中国稻田土壤中通过 n-damo 消耗约 0.91 Tg CH 和 2.17 Tg N。n-damo 活性和 n-damo 细菌群落结构的空间变化受土壤铵态氮含量、易降解有机碳含量和 pH 值的显著影响（p<0.05）。此外，不同气候带之间 n-damo 活性、细菌丰度和群落组成也存在显著差异。n-damo 活性与年平均空气温度呈正相关。综上所述，本研究结果表明 n-damo 在甲烷消耗和氮素去除方面对中国稻田具有重要意义，该过程受当地土壤和气候因素的调控。
