用抗癌胚抗原单克隆抗体F（ab'）2片段对结直肠癌进行免疫闪烁显像。

Immunoscintigraphy of colorectal carcinoma with F (ab')2 fragments of anti-CEA monoclonal antibody.

作者信息

Buraggi G, Callegaro L, Turrin A, Gennari L, Bombardieri E, Mariani G, Deleide G, Dovis M, Gasparini M, Doci R

出版信息

Cancer Detect Prev. 1987;10(5-6):335-45.

Abstract

A monoclonal antibody to carcinoembryonic antigen (CEA) (F023C5), belonging to IgG1 class, was obtained by cell fusion technique. Preliminary screening on different tissues was performed with immunoperoxidase staining, which showed good specificity of the antibody for gastric and colorectal carcinomas. F(ab')2 fragments were subsequently prepared and labeled with 131I and 111In. After immunoreactivity check the radiopharmaceuticals were injected intravenously. Sixteen patients with 22 primary or secondary localizations of colorectal carcinoma were studied following the recommendations of the ethical Committee of the Istituto Nazionale Tumori, Milan, Italy. Serial scans were performed after injection of the two radioactive reagents. In vivo pharmacokinetics of the compound was studied. Radioactivity level in surgical specimens was measured, and immunostaining was performed. All tumors were found to express the antigen. Eleven out of 12 tumor localizations of the gastrointestinal tract and three out of ten liver metastases were imaged. Specificity of tumor uptake was assessed by simultaneous injection of an irrelevant antibody.

摘要

通过细胞融合技术获得了一种针对癌胚抗原（CEA）的单克隆抗体（F023C5），它属于IgG1类。采用免疫过氧化物酶染色对不同组织进行了初步筛选，结果显示该抗体对胃癌和结直肠癌具有良好的特异性。随后制备了F(ab')2片段并用131I和111In进行标记。在检查免疫反应性后，将放射性药物静脉注射。按照意大利米兰国家肿瘤研究所伦理委员会的建议，对16例患有22处原发性或继发性结直肠癌定位的患者进行了研究。在注射两种放射性试剂后进行了系列扫描。研究了该化合物的体内药代动力学。测量了手术标本中的放射性水平，并进行了免疫染色。发现所有肿瘤均表达该抗原。12处胃肠道肿瘤定位中有11处以及10处肝转移中有3处被成像。通过同时注射无关抗体评估了肿瘤摄取的特异性。