Li Yangguang, Feng Yifan, Chen Shenyan, Li Renjie, Yang Yue, Guan Jing Jing, Ye Bang-Ce
Institute of Engineering Biology and Health, Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, 310014, Zhejiang, China.
Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, China.
Mikrochim Acta. 2022 Jun 10;189(7):250. doi: 10.1007/s00604-022-05341-2.
A stable ratiometric electrochemical sensor is introduced for the selective detection of carbendazim (CBD). Specifically, the proposed sensor employs a Co@MoC bimetallic nanomaterial as the glassy carbon electrode substrate and a layer of molecularly imprinted polymer (MIP) was in situ fabricated on glassy carbon electrode by electropolymerization, with o-aminophenol as the functional monomer and CBD acting as template. A ratiometric MIP sensor was constructed by adding ferrocene (Fc) internal reference directly to the sample solution. The bimetallic nanomaterials provide a large loading platform for the MIP layer through synergistic effects, amplifying the signal. Excellent CBD binding selectivity is achieved by the templating effect of the three-dimensional (3D) MIP layer. The internal standard is added directly to the electrolyte solution to be tested, allowing the new type of ratiometric electrochemical sensor to avoid the cumbersome steps of other methods and reducing the difficulty and human error of the experimental procedure. Combining a ratiometric strategy with a 3D MIP structure realises the dual-signal detection of CBD. The optimised sensor showed an excellent linear relationship between 0.01 and 1 000 μM, with a correlation coefficient of 0.997 and a detection limit of 3.4 nM (S/N = 3).
介绍了一种用于选择性检测多菌灵（CBD）的稳定比率型电化学传感器。具体而言，该传感器采用Co@MoC双金属纳米材料作为玻碳电极基底，并通过电聚合在玻碳电极上原位制备了一层分子印迹聚合物（MIP），以邻氨基酚为功能单体，CBD作为模板。通过将二茂铁（Fc）内参直接加入样品溶液中构建了比率型MIP传感器。双金属纳米材料通过协同效应为MIP层提供了一个大的负载平台，放大了信号。通过三维（3D）MIP层的模板效应实现了对CBD的优异结合选择性。将内标直接加入待测电解液中，使新型比率型电化学传感器避免了其他方法繁琐的步骤，降低了实验过程的难度和人为误差。将比率策略与3D MIP结构相结合实现了对CBD的双信号检测。优化后的传感器在0.01至1000μM之间呈现出优异的线性关系，相关系数为0.997，检测限为3.4 nM（S/N = 3）。
