Fukuda T, Hashimoto P H
Cell Tissue Res. 1987 Mar;247(3):555-64. doi: 10.1007/BF00215749.
The wall of the cerebral aqueduct was examined in 20 male rats at the light- and electron-microscopic levels. Disorder in ciliary orientation was occasionally seen in ordinary ependymal cells. Ependymal cells possessing intracellular cysts of 5 to 30 micron in diameter were observed within and beneath the aqueductal ependyma in all animals examined. Light-microscopic reconstruction from serial, 10-micron thick frontal sections revealed an extensive distribution of cystic ependymal cells (CECs), especially along the ependymal ridges in the rostral half of the aqueduct, and along the dorsal region of the aqueductal lining in the caudal half. Both cystic and surface membranes of CECs bore microvilli and cilia. Ectopic ependymal cells (EECs) characterized by densely packed microvilli, well-developed intermediate junctions and cilia, but without cysts, were situated in the subependymal region adjacent to a CEC or another EEC. The ependymal ridges were long, narrow and sporadically stratified ependymal linings extending rostrocaudally and bilaterally along the aqueductal surface. Tanycyte-like cells filled the surface region of the ridge, and CECs and EECs were frequently seen in the core. Intraventricularly injected microperoxidase was detected among densely packed microvilli but not in the cystic lumina of CECs, indicating that EECs and CECs are distinct entities.
在20只雄性大鼠中，对大脑导水管壁进行了光镜和电镜水平的检查。普通室管膜细胞偶尔可见纤毛方向紊乱。在所有检查的动物中，在导水管室管膜内及其下方观察到直径为5至30微米的细胞内囊肿的室管膜细胞。从10微米厚的连续额状切片进行光镜重建显示，囊性室管膜细胞（CEC）广泛分布，尤其是在导水管前半部分的室管膜嵴处，以及后半部分导水管内衬的背侧区域。CEC的囊性膜和表面膜都有微绒毛和纤毛。异位室管膜细胞（EEC）的特征是微绒毛密集、中间连接发达且有纤毛，但无囊肿，位于与CEC或另一个EEC相邻的室管膜下区域。室管膜嵴是沿着导水管表面向头尾和双侧延伸的长而窄且偶尔分层的室管膜内衬。类似伸展细胞的细胞充满了嵴的表面区域，在核心部位经常可见CEC和EEC。脑室内注射的微过氧化物酶在密集排列的微绒毛中被检测到，但在CEC的囊性腔隙中未检测到，这表明EEC和CEC是不同的实体。
