Wang Luokai, Ju Xiaochen, Cong Yu, Lin Hong, Wang Jingxue
Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao 266003, China.
Foods. 2022 May 27;11(11):1578. doi: 10.3390/foods11111578.
Endolysins are enzymes used by bacteriophages to cleave the host cell wall in the final stages of the lytic cycle. As such, they are considered promising antibacterial agents for controlling and combating multidrug-resistant (MDR) bacteria. However, the application of endolysins targeting Gram-negative bacteria is greatly hindered by the outer membrane on these bacteria. Lysqdvp001, an endolysin with modular structure, has been reported as one of the most efficient endolysins against the Gram-negative bacterium . In this study, Plychap001, the truncated recombinant catalytic domain of Lysqdvp001, was demonstrated to exhibit a direct and efficient bactericidal activity against broad spectrum of strains. Plychap001 was shown to be highly stable and retain high bactericidal activity at high temperatures, over a wide pH range, and at high NaCl concentrations. Plychap001 also exhibited a synergistic lytic effect with EDTA. Additionally, Plychap001 was found to efficiently degrade and eliminate biofilms on polystyrene surfaces. Our study establishes Plychap001 as a promising method for controlling in the food industry.
内溶素是噬菌体在裂解周期的最后阶段用于裂解宿主细胞壁的酶。因此，它们被认为是控制和对抗多重耐药（MDR）细菌的有前景的抗菌剂。然而，靶向革兰氏阴性菌的内溶素的应用受到这些细菌外膜的极大阻碍。Lysqdvp001是一种具有模块化结构的内溶素，已被报道为对抗革兰氏阴性菌最有效的内溶素之一。在本研究中，Lysqdvp001的截短重组催化结构域Plychap001被证明对多种菌株具有直接且高效的杀菌活性。Plychap001在高温、宽pH范围和高NaCl浓度下表现出高度稳定性并保持高杀菌活性。Plychap001还与EDTA表现出协同裂解作用。此外，发现Plychap001能有效降解和消除聚苯乙烯表面的生物膜。我们的研究将Plychap001确立为食品工业中控制[此处原文缺失相关细菌名称]的一种有前景的方法。
