Romero Jaime, Blas-Chumacero Sergueia, Urzúa Victoria, Villasante Alejandro, Opazo Rafael, Gajardo Felipe, Miranda Claudio D, Rojas Rodrigo
Laboratorio de Biotecnología de Alimentos, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, El Líbano 5524, Santiago 7830489, Chile.
Laboratorio de Patobiología Acuática, Departamento de Acuicultura, Universidad Católica del Norte, Larrondo 1281, Coquimbo 1780000, Chile.
Microorganisms. 2024 Apr 30;12(5):904. doi: 10.3390/microorganisms12050904.
species are naturally found in estuarine and marine ecosystems, but are also recognized as significant human enteropathogens, often linked to seafood-related illnesses. In aquaculture settings, Vibrio poses a substantial risk of infectious diseases, resulting in considerable stock losses and prompting the use of antimicrobials. However, this practice contributes to the proliferation of antimicrobial-resistant (AMR) bacteria and resistance genes. Our investigation aimed to explore the potential of biological agents such as bacteriophage CH20 and endolysin LysVPp1 in reducing Vibrio bacterial loads in both rotifer and fish larvae. LysVPp1's lytic activity was assessed by measuring absorbance reduction against various pathogenic Vibrio strains. Phage CH20 exhibited a limited host range, affecting only GV09, a highly pathogenic strain. Both CH20 and LysVPp1 were evaluated for their effectiveness in reducing Vibrio load in rotifers or fish larvae through short-setting bioassays. Our results demonstrated the significant lytic effect of endolysin LysVPp1 on strains of , , and . Furthermore, we have showcased the feasibility of reducing the load of pathogenic Vibrio in live feed and fish larvae by using a non-antibiotic-based approach, such as lytic phage and endolysin LysVPp1, thus contributing to the progress of a sustainable aquaculture from a One Health perspective.
弧菌属物种自然存在于河口和海洋生态系统中，但也被认为是重要的人类肠道病原体，常与海鲜相关疾病有关。在水产养殖环境中，弧菌会带来重大的传染病风险，导致大量养殖生物死亡，并促使人们使用抗菌药物。然而，这种做法会导致抗菌药物耐药（AMR）细菌和耐药基因的扩散。我们的研究旨在探索诸如噬菌体CH20和内溶素LysVPp1等生物制剂在降低轮虫和鱼苗中弧菌细菌载量方面的潜力。通过测量针对各种致病性弧菌菌株的吸光度降低来评估LysVPp1的裂解活性。噬菌体CH20的宿主范围有限，仅影响高致病性菌株GV09。通过短期生物测定评估了CH20和LysVPp1在降低轮虫或鱼苗中弧菌载量方面的有效性。我们的结果证明了内溶素LysVPp1对哈维氏弧菌、溶藻弧菌和副溶血弧菌菌株具有显著的裂解作用。此外，我们展示了通过使用基于非抗生素的方法，如裂解噬菌体和内溶素LysVPp1，来降低活饵和鱼苗中致病性弧菌载量的可行性，从而从“同一健康”的角度推动可持续水产养殖的发展。
