Onsa-Ard Amnart, Thongboontho Rungthip, Munkong Narongsuk, Phromnoi Kanokkarn, Ontawong Atcharaporn, Pengnet Sirinat, Thim-Uam Arthid
Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand.
Department of Pathology, School of Medicine, University of Phayao, Phayao 56000, Thailand.
Foods. 2022 May 30;11(11):1622. doi: 10.3390/foods11111622.
Type I interferons (IFNs-I) are inflammatory cytokines that play an essential role in the pathogenesis of inflammation and autoimmune diseases. Signaling through nucleic acid sensors causes the production of IFNs-I. A stimulator of interferon genes (STING) is a DNA sensor that signals transduction, leading to the production of IFNs-I after their activation. This study aims to determine the anti-inflammatory effects of red rice bran extract (RRBE) on macrophages through the activation of STING signaling. RAW264.7 macrophage cells were stimulated with STING agonist (DMXAA) with and without RRBE. Cells and supernatant were collected. The level of mRNA expression was determined by qPCR, and inflammatory cytokine production was investigated by ELISA. The results indicate that RRBE significantly lowers the transcription of STING and interferon-stimulated genes (ISGs). Moreover, RRBE suppresses the phosphorylation of STING, leading to a decrease in the expression of , a transcription factor that initiates IFN-I signaling. Our results provide evidence that red rice bran extract may be a protective compound for inflammatory diseases by targeting STING signaling.
I型干扰素（IFNs-I）是一类炎症细胞因子，在炎症和自身免疫性疾病的发病机制中发挥着重要作用。通过核酸传感器发出信号会导致IFNs-I的产生。干扰素基因刺激物（STING）是一种DNA传感器，负责信号转导，在其激活后导致IFNs-I的产生。本研究旨在通过激活STING信号来确定红米糠提取物（RRBE）对巨噬细胞的抗炎作用。用STING激动剂（DMXAA）刺激RAW264.7巨噬细胞，同时添加或不添加RRBE。收集细胞和上清液。通过qPCR测定mRNA表达水平，并通过ELISA研究炎症细胞因子的产生。结果表明，RRBE显著降低了STING和干扰素刺激基因（ISGs）的转录。此外，RRBE抑制STING的磷酸化，导致启动IFN-I信号的转录因子的表达减少。我们的结果提供了证据，表明红米糠提取物可能通过靶向STING信号成为炎症性疾病的一种保护性化合物。
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