Hernandis Verónica, Escudero Elisa, Galecio Juan Sebastián, Marín Pedro
Department of Pharmacology, Faculty of Veterinary Medicine, University of Murcia, 30100 Murcia, Spain.
Escuela de Medicina Veterinaria, Colegio de Ciencias de la Salud, Universidad San Francisco de Quito, Cumbayá 170157, Ecuador.
Animals (Basel). 2022 May 27;12(11):1385. doi: 10.3390/ani12111385.
Tylvalosin (TV) is a macrolide antibiotic that is used for treating respiratory and enteric bacterial infections in swine and in poultry. In the coming years, the use of this drug will probably be widely studied in different species, but before its use in each veterinary species, macrolide analytical determination in various biological fluids is a pre-requisite step for the rational dose calculation of TV based on specific pharmacokinetic information. Its quantification is essential for detecting and avoiding the appearance of residues in animal products intended for human consumption. Therefore, a robust chromatographic method coupled with an ultraviolet detector was fully validated for the quantification of TV in pig plasma. A mixture (78:22) of (A) 0.3% formic acid in water and (B) acetonitrile was used as the mobile phase. TV and enrofloxacin (internal standard) were eluted at 14.1 and 5.9 min, respectively. Calibration curves ranged from 0.1 to 5 μg/mL. The accuracy and precision parameters for the quality controls were always <13.0%. Recovery ranged from 89.66 to 96.92%. The detection and quantification limits were found to be 0.05 μg/mL and 0.1 μg/mL, respectively. This method could be applied to develop pharmacokinetic studies.
替米考星（TV）是一种大环内酯类抗生素，用于治疗猪和家禽的呼吸道及肠道细菌感染。在未来几年，这种药物可能会在不同物种中得到广泛研究，但在用于每种兽医物种之前，根据特定的药代动力学信息对各种生物流体中的大环内酯进行分析测定是合理计算替米考星剂量的前提步骤。其定量对于检测和避免供人类食用的动物产品中出现残留至关重要。因此，一种结合紫外检测器的稳健色谱方法已完全验证用于猪血浆中替米考星的定量。以（A）0.3%甲酸水溶液和（B）乙腈按78:22的比例混合作为流动相。替米考星和恩诺沙星（内标）分别在14.1分钟和5.9分钟洗脱。校准曲线范围为0.1至5μg/mL。质量控制的准确度和精密度参数始终<13.0%。回收率范围为89.66%至96.92%。检测限和定量限分别为0.05μg/mL和0.1μg/mL。该方法可用于开展药代动力学研究。
