采用高效液相色谱法测定儿科样本中的甲氧苄啶。

Determination of trimethoprim in pediatric samples by high-performance liquid chromatography.

作者信息

Hoppu K, Arjomaa P

出版信息

Clin Chim Acta. 1987 Feb 27;163(1):81-6. doi: 10.1016/0009-8981(87)90036-2.

Abstract

We describe and evaluate modifications to a known high-performance liquid chromatography assay of trimethoprim. The sensitivity, specificity and wide range of linearity (0.11-690 mumol/l) of this method make it suitable for analysing samples with large variations in volume and concentration. We have used it to study the pharmacokinetics of trimethoprim in children, including newborns. Automated injection is a useful option if the sample volume is not limited. The recovery (at 3.44 mumol/l) of the method was 99.8% with a coefficient of variation of 6.8% for manually injected samples. The corresponding results for automated injection were 97.9% and 12.8%.

摘要

我们描述并评估了对已知的甲氧苄啶高效液相色谱分析方法的改进。该方法的灵敏度、特异性以及宽线性范围（0.11 - 690 μmol/L）使其适用于分析体积和浓度变化较大的样本。我们已将其用于研究包括新生儿在内的儿童中甲氧苄啶的药代动力学。如果样本体积不受限制，自动进样是一个有用的选择。对于手动进样的样本，该方法在3.44 μmol/L时的回收率为99.8%，变异系数为6.8%。自动进样的相应结果分别为97.9%和12.8%。