Institute of Biomedical Engineering and Technology, Academy for Engineering and Technology, Fudan University, Shanghai 200433, China.
Department of Optical Science and Engineering, School of Information Science and Technology, Fudan University, Shanghai 200433, China.
Molecules. 2022 Jun 2;27(11):3582. doi: 10.3390/molecules27113582.
With the development of precision medicine, antigen/antibody-targeted therapy has brought great hope to tumor patients; however, the migration of tumor cells, especially a small number of cells flowing into blood or other tissues, remains a clinical challenge. In particular, it is difficult to use functional gold nanomaterials for targeted clinical tumor diagnosis while simultaneously obtaining stable and highly sensitive Raman signals. Therefore, we developed a detection method for functional Au Nanostars (AuNSs) with dual signal enhancement that can specifically track location and obtain high-intensity surface-enhanced Raman scattering (SERS) signals. First, AuNSs with specific optical properties were synthesized and functionalized. The Raman dye 4-mercapto-hydroxybenzoic acid and polyethylene glycol were coupled with the tumor marker, epidermal growth factor receptor, to obtain the targeted SERS probes. In addition, a detection chip was prepared for Raman detection with physical enhancement, exhibiting a 40-times higher signal intensity than that of quartz glass. This study combines physical enhancement and SERS enhancement technologies to achieve dual enhancement, enabling the detection of a highly sensitive and stable Raman signal; this has potential clinical value for antigen/antibody-targeted tumor diagnosis and treatment.
随着精准医学的发展，抗原/抗体靶向治疗为肿瘤患者带来了很大的希望；然而，肿瘤细胞的迁移，特别是少量细胞流入血液或其他组织，仍然是一个临床挑战。特别是，很难同时使用功能金纳米材料进行靶向临床肿瘤诊断，同时获得稳定和高灵敏度的拉曼信号。因此，我们开发了一种具有双重信号增强功能的 Au Nanostars（AuNSs）检测方法，可以特异性地跟踪位置并获得高强度的表面增强拉曼散射（SERS）信号。首先，合成并功能化具有特定光学特性的 AuNSs。将拉曼染料 4-巯基-羟基苯甲酸和聚乙二醇与肿瘤标志物表皮生长因子受体偶联，得到靶向 SERS 探针。此外，还制备了用于拉曼检测的检测芯片，具有物理增强，信号强度比石英玻璃高 40 倍。本研究结合物理增强和 SERS 增强技术实现双重增强，能够检测到高灵敏度和稳定的拉曼信号；这对基于抗原/抗体的肿瘤诊断和治疗具有潜在的临床价值。
