Wang Huanhuan, Lu Shan, Guan Xiangyu, Jiang Yuan, Wang Bin, Hua Jian, Zou Baohong
The State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.
Plant Biology Section, School of Integrated Plant Science, Cornell University, Ithaca, NY, United States.
Front Plant Sci. 2022 May 24;13:851731. doi: 10.3389/fpls.2022.851731. eCollection 2022.
The ()/ () genes are key regulators of cold acclimation and freezing tolerance in the chilling tolerant . Here, we investigated the function of three members of the 10 rice genes, , , and , in the chilling sensitive rice plants. Their loss of function (LOF) mutants were each more chilling susceptible compared to the wild type, and the LOF mutants of all three genes, , were more chilling susceptible than any of the single mutants. Strikingly, these mutants were capable of cold acclimation, indicating that these rice genes are important for basal chilling tolerance but not cold acclimation. Transcriptome and physiology analyses suggest that the // genes are involved in reactive oxygen species (ROS) scavenging and cell death regulation under chilling. Furthermore, these three rice genes are found to promote tolerance to other abiotic stresses: the // genes are positive regulators of heat tolerance, and are positive regulators of salt tolerance, and is a positive regulator of drought tolerance. These findings expand our knowledge of the roles of DREB1 proteins in plants, enhance our mechanistic understanding of abiotic stress tolerance and will facilitate the generation of stress-tolerant crop plants.
（）/（）基因是耐冷植物中冷驯化和耐冻性的关键调节因子。在此，我们研究了10个水稻（）基因中的三个成员，即、和，在冷敏感水稻植株中的功能。与野生型相比，它们的功能缺失（LOF）突变体对冷更敏感，并且这三个基因（）的LOF突变体比任何单个突变体对冷更敏感。引人注目的是，这些突变体能够进行冷驯化，这表明这些水稻（）基因对基础耐冷性很重要，但对冷驯化不重要。转录组和生理学分析表明，//基因参与了冷胁迫下活性氧（ROS）清除和细胞死亡调控。此外，发现这三个水稻（）基因能促进对其他非生物胁迫的耐受性：//基因是耐热性的正调节因子，和是耐盐性的正调节因子，并且是耐旱性的正调节因子。这些发现扩展了我们对植物中DREB1蛋白作用的认识，增强了我们对非生物胁迫耐受性机制的理解，并将有助于培育耐胁迫作物。
