Smolik Miłosz, Ochmian Ireneusz, Bobrowska-Chwat Aleksandra, Chwat Gerard, Arus Liina, Banaszczak Piotr, Bocianowski Jan, Milczarski Paweł, Ostrowska Krystyna
Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology in Szczecin, Słowackiego 17, 71-434 Szczecin, Poland.
Department of Horticulture, West Pomeranian University of Technology in Szczecin, Słowackiego 17, 71-434 Szczecin, Poland.
Biotechnol Rep (Amst). 2022 Mar 23;34:e00721. doi: 10.1016/j.btre.2022.e00721. eCollection 2022 Jun.
Due to its value and economic importance, the genome of L. has been widely studied in various fields of science. In this study the genetic structure and relationships between 24 accessions of of different origins were assessed. A total of 692, 814, and 258 loci were amplified using 43 RAPD (random amplified polymorphic DNA), 40 ISSR (intersimple sequence repeat), and 20 R-ISSR (RAPD+ISSR) primers, respectively. Among the amplified loci, 66-78% were polymorphic and 12-20% were private. Selected R-ISSR sequences were detected in transcripts. Cluster and STRUCTURE analyses performed for each of the techniques revealed the existing differences and unknown similarities between the genotypes. The -factor values calculated in the Mantel test indicated highly significant positive correlations between the Nei distance matrices, similar to the values ( = 0.223, = 0.279, = 0.363) determined in the analysis of molecular variance. It was found that 78%, 72%, and 64% of the genetic variations were related to the differences observed within the populations, which suggest that the variations are mainly reflected in the differences among the genotypes. The principal coordinate analysis showed greater differences between the mean distances of the genotype pair and the actual distances of the same pairs on the Nei matrix compared to multidimensional scaling. These differences were 45%, 56%, and 42% higher for RAPD, ISSR and R-ISSR, respectively.
由于其价值和经济重要性，L.的基因组已在各个科学领域得到广泛研究。在本研究中，评估了24份不同来源的L.的遗传结构和关系。分别使用43个RAPD（随机扩增多态性DNA）、40个ISSR（简单序列重复区间）和20个R-ISSR（RAPD+ISSR）引物扩增出总共692、814和258个位点。在扩增出的位点中，66%-78%是多态性的，12%-20%是私有位点。在转录本中检测到了选定的R-ISSR序列。对每种技术进行的聚类和STRUCTURE分析揭示了基因型之间存在的差异和未知的相似性。Mantel检验中计算的r值表明Nei距离矩阵之间存在高度显著的正相关，类似于分子方差分析中确定的r值（r = 0.223，r = 0.279，r = 0.363）。发现78%、72%和64%的遗传变异与群体内观察到的差异有关，这表明变异主要体现在基因型之间的差异上。主坐标分析表明，与多维标度相比，L.基因型对的平均距离与Nei矩阵上相同对的实际距离之间的差异更大。这些差异在RAPD、ISSR和R-ISSR中分别高出45%、56%和42%。
