苏丹肾移植受者巨细胞病毒的分子检测和糖蛋白 B（UL55）基因分型。

Molecular Detection and Glycoprotein B (UL55) Genotyping of Cytomegalovirus among Sudanese Renal Transplant Recipients.

机构信息

Department of Microbiology, College of Medical Laboratory Science, Sudan University of Science and Technology, Sudan.

Biochemistry Department, Faculty of Sciences, King Abdulaziz University, Jeddah 21452, Saudi Arabia.

出版信息

Biomed Res Int. 2022 May 31;2022:5403694. doi: 10.1155/2022/5403694. eCollection 2022.

DOI:10.1155/2022/5403694

PMID:35686228

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9173907/

Abstract

BACKGROUND

Cytomegalovirus (CMV) is the most common opportunistic pathogen among renal transplants with significant morbidity and mortality. This study was designed to detect CMV DNA and to determine the frequency of different glycoprotein B (UL55) genotypes among Sudanese renal transplant recipients.

METHODS

One hundred and four renal transplant recipients were included in this study. A blood specimen was collected from each recipient. DNA was extracted from plasma using the QIAamp DNA mini kit. CMV amplification and quantification were performed using CMV Real-RT Quant kits. Genotyping of human CMV was carried out by nested PCR and sequencing of the highly diverse region of .

RESULTS

CMV DNA was detected in 40/104 (38.5%) of renal transplant recipients. The average of the CMV DNA viral load was 358 × 10 copies/ml (6.5 log) ranging from 62 copies/ml (1.8 log) to 1.43 × 10 copies/ml (9 log). CMV viremia was detected in 60% of recipients of less than 1-12 months, 17% of 13-24, 10% of 25-36, 5% of 37-48, and 8% in more than 48 months posttransplantation with no association ( = 0.296) between CMV viremia and postrenal transplantation time. The association between the type of immunosuppressive drugs and high viral loads (>1000 copies/ml) showed a significant difference ( = 0.05). The association between CMV loads of >1000 copies/ml and symptoms of CMV disease was highly significant ( ≤ 0.001). Fever 7 (41%), fever and leucopenia 6 (35%), and gastrointestinal disease 4 (24%) were the most common symptoms of CMV disease. CMV genotyping revealed 8 cases (80%) for 3 and 2 cases (20%) for 4 genotypes. The most frequent genotype among Sudanese renal transplant recipients was 3.

CONCLUSIONS

The frequency of CMV DNA is high among Sudanese renal transplant recipients. CMV 3 is the most predominant glycoprotein B genotype in Sudanese renal transplant recipients.

摘要

背景

巨细胞病毒（CMV）是肾移植患者中最常见的机会性病原体，具有显著的发病率和死亡率。本研究旨在检测苏丹肾移植受者的 CMV DNA，并确定不同糖蛋白 B（UL55）基因型的频率。

方法

本研究纳入 104 例肾移植受者。采集每位受者的血样。使用 QIAamp DNA mini 试剂盒从血浆中提取 DNA。使用 CMV Real-RT Quant 试剂盒进行 CMV 扩增和定量。通过巢式 PCR 和高变区的测序进行人类 CMV 基因分型。

结果

在 104 例肾移植受者中，有 40 例（38.5%）检测到 CMV DNA。CMV DNA 病毒载量的平均值为 358×10 拷贝/ml（6.5 log），范围为 62 拷贝/ml（1.8 log）至 1.43×10 拷贝/ml（9 log）。在移植后 1-12 个月、13-24 个月、25-36 个月、37-48 个月和超过 48 个月的受者中，分别有 60%、17%、10%、5%和 8%检测到 CMV 血症，CMV 血症与肾移植后时间之间无相关性（=0.296）。免疫抑制药物的类型与高病毒载量（>1000 拷贝/ml）之间存在显著差异（=0.05）。CMV 病毒载量>1000 拷贝/ml 与 CMV 疾病症状之间存在高度显著相关性（≤0.001）。CMV 疾病最常见的症状为发热 7 例（41%）、发热伴白细胞减少 6 例（35%）和胃肠道疾病 4 例（24%）。CMV 基因分型显示 8 例（80%）为 3 型，2 例（20%）为 4 型。苏丹肾移植受者中最常见的基因型是 3 型。