Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan.
Chem Commun (Camb). 2022 Jun 30;58(53):7368-7371. doi: 10.1039/d2cc02159e.
The capture and detection of cells expressing a breast-cancer related membrane protein, namely a BT474 cell line expressing HER2, is demonstrated using ion-sensitive field effect transistors (ISFETs). BT474 cells were exposed to anti-HER2 antibodies and urease-conjugated secondary antibodies to induce chemical signal amplification by adding urea.
使用离子敏感场效应晶体管（ISFET），可以捕获并检测表达乳腺癌相关膜蛋白的细胞，例如表达 HER2 的 BT474 细胞系。BT474 细胞暴露于抗 HER2 抗体和脲酶结合的二级抗体，通过添加尿素来诱导化学信号放大。
