Enzyme Technology Research Team, Biorefinery Technology and Bioproduct Research Group, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Phahonyothin Road, Khlong Luang, Pathumthani, Thailand.
Biosci Biotechnol Biochem. 2022 Jul 22;86(8):1144-1150. doi: 10.1093/bbb/zbac091.
Schizophyllum commune is a mushroom-forming fungus well-known for its ability to degrade lignocellulosic materials and production of schizophyllan, a high added-value product for cosmeceutical, pharmaceutical, and biomaterial industries. Conventionally, schizophyllan is produced by submerged fermentation using glucose as a carbon source. In this work, we demonstrate that alkaline pretreated bagasse can be used by Schizophyllum commune as an alternative carbon source for the production of schizophyllan. The influence of different factors was investigated including cultivation time, biomass loading, and culturing media component and a co-product correlation model was proposed. In this lab-scale study, a yield of 4.4 g/L of schizophyllan containing 89% glucose was achieved. In addition to schizophyllan, the cellulolytic enzymes co-produced during this process were isolated and characterized and could find applications in a range of industrial processes. This demonstrates the potential of using agricultural waste as a cheaper alternative feedstock for this biorefinery process.
裂褶菌是一种具有降解木质纤维素能力的蕈菌类真菌，以其能够生产裂褶多糖而闻名，裂褶多糖是化妆品、制药和生物材料行业的高附加值产品。传统上，裂褶多糖是通过使用葡萄糖作为碳源的深层发酵生产的。在这项工作中，我们证明碱性预处理的甘蔗渣可以被裂褶菌用作生产裂褶多糖的替代碳源。研究了不同因素的影响，包括培养时间、生物量负荷和培养介质成分，并提出了一个副产物相关模型。在这个实验室规模的研究中，裂褶菌的产量达到了 4.4 g/L，其中含有 89%的葡萄糖。除了裂褶多糖，在这个过程中共同产生的纤维素酶也被分离和表征，它们可以在一系列工业过程中得到应用。这表明了利用农业废物作为这种生物炼制过程更廉价的替代原料的潜力。
