Jamshidian Hajar, Shojaosadati Seyed Abbas, Vilaplana Francisco, Mousavi Seyed Mohammad, Soudi Mohammad Reza
Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University, 14115-143 Tehran, Iran.
Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University, 14115-143 Tehran, Iran.
Int J Biol Macromol. 2016 Nov;92:484-493. doi: 10.1016/j.ijbiomac.2016.07.059. Epub 2016 Jul 18.
This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production. Initially, one factor-at-a-time method was used to find the best carbon and nitrogen sources for schizophyllan production. Subsequently, response surface methodology was employed to optimize the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture. Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively. Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues. Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
本研究证明了裂褶菌ATCC 38548能有效利用低成本的农业底物，特别是枣糖浆来生产裂褶多糖。最初，采用一次一个因素的方法来寻找裂褶多糖生产的最佳碳源和氮源。随后，运用响应面法优化培养基成分水平，以在深层培养中最大化底物转化率和裂褶多糖产量。当枣糖浆和玉米浆的浓度、接种量和搅拌速度分别为7.02%w/v、0.10%w/v、7.68%v/v和181rpm时，获得了最大产物产量（0.12克裂褶多糖/克枣糖浆）和裂褶多糖产量（8.5克/升）。糖成分分析、傅里叶变换红外光谱、核磁共振和摩尔质量测定揭示了从枣糖浆中回收的裂褶多糖的纯度和分子特性，糖苷键分析显示有三个主要的裂褶多糖特征峰，分别来自3-连接、3,6-连接和末端葡萄糖残基。最后，工艺经济分析表明，与蔗糖和麦芽提取物等传统碳源和氮源相比，使用枣糖浆和玉米浆作为营养物质将使裂褶多糖生产的原材料成本降低约6倍。
