Jensen S, Sørensen T, Zimmer J
Cryobiology. 1987 Apr;24(2):120-34. doi: 10.1016/0011-2240(87)90014-9.
Intracerebral grafting of immature brain tissue is now widely used as a tool to study neuronal development and regeneration in the brain and spinal cord. This has stimulated the interest in methods for storage of such tissue before transplantation. In this study a method for cryopreservation of immature rat central nervous tissue is presented and discussed in relation to current cryobiological principles. The method was applied to brain tissue from 16- and 17-day-old fetal rats, including the neocortex, habenula, septum and basal forebrain, cerebellum, and retina. After storage in liquid nitrogen from 6 to 52 days the tissue was grafted into the brain of adult rats. The recipients survived for 23 to 673 days before their brains were processed by current neuroanatomical, histological methods. The presence of graft tissues was recorded and their cellular and connective organization was examined, including their exchange of nerve connections with the host brain. The results obtained were comparable with results from other studies where the same tissues were grafted immediately after removal from the donor, and a study of cryopreservation of developing hippocampal tissue. We conclude that cryopreservation is a reliable method for storage of immature neural tissue later to be used for intracerebral grafting.
未成熟脑组织的脑内移植如今被广泛用作研究大脑和脊髓中神经元发育与再生的工具。这激发了人们对移植前此类组织保存方法的兴趣。在本研究中，提出了一种未成熟大鼠中枢神经组织的冷冻保存方法，并结合当前的低温生物学原理进行了讨论。该方法应用于16日龄和17日龄胎鼠的脑组织，包括新皮层、缰核、隔区和基底前脑、小脑以及视网膜。在液氮中保存6至52天后，将组织移植到成年大鼠的大脑中。受体存活23至673天后，用当前的神经解剖学、组织学方法处理其大脑。记录移植组织的存在情况，并检查其细胞和结缔组织构成，包括它们与宿主大脑的神经连接交换情况。所获得的结果与其他研究结果相当，其他研究中相同组织从供体取出后立即进行移植，以及一项关于发育中的海马组织冷冻保存的研究。我们得出结论，冷冻保存是一种可靠的方法，可用于保存后来用于脑内移植的未成熟神经组织。
