McLean M P, Miller J B
Cytobios. 1987;49(197):73-84.
Although oestradiol is luteotropic in the rabbit, the mechanism through which oestradiol maintains the corpus luteum is unknown. To determine if ultrastructural changes in the rabbit corpus luteum accompany oestradiol treatment, corpora lutea were removed on day 10 of pseudopregnancy from hysterectomized rabbits treated with oestradiol implants and prepared for electron microscopy. Morphometric analysis was performed on luteal cells in each group (seven animals/group, four micrographs/animal) and organelle profile volumes, estimated by point-counting techniques, were expressed as a percentage of the cell volume. Morphometric analysis revealed no significant differences in nuclear, mitochondrial, lysosomal, Golgi or endoplasmic reticular volumes between control and oestradiol-treated animals. In contrast, lipid droplet volume was significantly elevated (p less than 0.001) in oestradiol-treated animals. This data suggests that an important function of oestradiol in the rabbit corpus luteum is to regulate the uptake and storage of cholesterol presumably for progesterone production.
虽然雌二醇对兔子具有促黄体生成作用，但其维持黄体的机制尚不清楚。为了确定雌二醇处理兔子黄体时是否伴有超微结构变化，在假孕第10天，从接受雌二醇植入物治疗的子宫切除兔子中取出黄体，并制备用于电子显微镜检查的样本。对每组黄体细胞进行形态计量分析（每组7只动物，每只动物4张显微照片），通过点计数技术估计的细胞器轮廓体积以细胞体积的百分比表示。形态计量分析显示，对照组和雌二醇处理组动物的细胞核、线粒体、溶酶体、高尔基体或内质网体积没有显著差异。相比之下，雌二醇处理组动物的脂滴体积显著增加（p小于0.001）。该数据表明，雌二醇在兔子黄体中的一个重要功能是调节胆固醇的摄取和储存，可能用于孕酮的产生。
