Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, PR China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Int J Syst Evol Microbiol. 2022 Jun;72(6). doi: 10.1099/ijsem.0.005416.
A novel ligninase-producing actinomycete, designated strain NEAU-G4, was isolated from a soil sample and subjected to a polyphasic taxonomic study to establish its status. According to 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus , with the highest sequence similarity to DSM 44496 (99.2 %). The whole-cell sugars contained galactose and arabinose. The amino acid of the cell wall was determined to be -diaminopimelic acid. The major fatty acids (>10 %) were C, C 9, C and C 7. The predominant menaquinone was identified as MK-8(H, ω-cycl). The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. Strain NEAU-G4 had a draft genome size of 6 405 167 bp, annotated with 5815 protein-coding genes. The DNA G+C content was 67.6 mol%. Phylogenetic analysis using the 16S rRNA gene and whole-genome sequences showed that strain NEAU-G4 formed a stable phyletic line with DSM 44496. The digital DNA-DNA hybridization and average nucleotide identity values between them were 63.7 % (60.8-66.5 %) and 95.5 %, respectively. Moreover, genomic analysis indicated that strain NEAU-G4 had the potential to degrade lignin and produce bioactive compounds. On the basis of genotypic analysis, physiological data, as well as phenotypic and chemotaxonomic characterizations, it is concluded that the organism be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-G4 (=CCTCC AA 2020038=DSM 111936).
一株新型产木聚糖酶放线菌，命名为 NEAU-G4 菌株，从土壤样本中分离出来，并进行了多相分类学研究，以确定其地位。根据 16S rRNA 基因序列比较，该分离物被鉴定为属的成员，与 DSM 44496 的序列相似度最高（99.2%）。全细胞糖含有半乳糖和阿拉伯糖。细胞壁的氨基酸被确定为 -二氨基庚二酸。主要脂肪酸（>10%）为 C 、 C 9 、 C 和 C 7 。主要的menaquinone 被鉴定为 MK-8（H，ω-环）。主要的极性脂类由双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰肌醇组成。NEAU-G4 菌株的基因组草图大小为 6405167bp，注释了 5815 个蛋白质编码基因。DNA G+C 含量为 67.6mol%。基于 16S rRNA 基因和全基因组序列的系统发育分析表明，NEAU-G4 菌株与 DSM 44496 形成了一个稳定的进化分支。它们之间的数字 DNA-DNA 杂交和平均核苷酸同一性值分别为 63.7%（60.8-66.5%）和 95.5%。此外，基因组分析表明，NEAU-G4 菌株具有降解木质素和产生生物活性化合物的潜力。基于基因型分析、生理数据以及表型和化学分类特征，该生物被归类为代表一个新的属种，命名为 sp. nov.。模式菌株为 NEAU-G4（=CCTCC AA 2020038=DSM 111936）。
