Yang Yanming, Qian Lulu, Lin Jiaying, Qi Cuiping, Zhao Tianxin, Wang Xiangjing, Zhao Junwei, Xiang Wensheng
Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, PR China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Int J Syst Evol Microbiol. 2022 Jun;72(6). doi: 10.1099/ijsem.0.005420.
A Gram-positive, cellulose-degrading actinobacterium, designed strain NEAU-YM18, was isolated from rhizosphere soil of wheat ( L.) sampled in Langfang, Hebei Province, PR China. The novel strain was characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics confirmed that strain NEAU-YM18 belonged to the genus . Cells of strain NEAU-YM18 were observed to contain - and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids. The acyl type of the cell-wall muramic acid was glycolyl. The whole-cell hydrolysates were xylose, glucose and ribose. The phospholipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were iso-C, iso-C, C 9 and summed feature 5 (anteiso-C/C 6,9). The menaquinones were MK-9(H), MK-9(H) and MK-9(H). The DNA G+C content was 71.1 %. The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18 was closely related to 2-25(1) (98.4 % 16S rRNA gene sequence similarity), NEAU-JM1 (98.3%) and H14505 (98.3 %) and formed a branch with H14505. Furthermore, the whole genome phylogeny of strain NEAU-YM18 showed that the strain formed an independent clade. The digital DNA-DNA hybridization results between NEAU-YM18 and 2-25(1), NEAU-JM1 and H14505 were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively. These genetic results and some phenotypic characteristics could distinguish strain NEAU-YM18 from its reference strains. In addition, genomic analysis confirmed that strain NEAU-YM18 had the potential to decompose cellulose and produce bioactive compounds. Therefore, strain NEAU-YM18 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-YM18 (=CCTCC AA 2020040=JCM 33977).
从中国河北省廊坊市采集的小麦根际土壤中分离出一株革兰氏阳性、可降解纤维素的放线菌，命名为菌株NEAU-YM18。采用多相分类法对该新菌株进行了表征。形态学和化学分类学特征证实菌株NEAU-YM18属于该属。观察到菌株NEAU-YM18的细胞含有作为诊断性细胞壁氨基酸的L-和3-羟基二氨基庚二酸。细胞壁胞壁酸的酰基类型为糖基。全细胞水解产物为木糖、葡萄糖和核糖。磷脂由二磷脂酰甘油、磷脂酰乙醇胺和磷脂酰肌醇组成。主要脂肪酸为异-C15:0、异-C16:0、C19:0和总和特征5（anteiso-C17:0/C19:0）。甲基萘醌为MK-9(H4)、MK-9(H6)和MK-9(H8)。DNA G+C含量为71.1%。16S rRNA基因序列和系统发育分析结果表明，菌株NEAU-YM18与Streptomyces sp. 2-25(1)（16S rRNA基因序列相似性为98.4%）、Streptomyces sp. NEAU-JM1（98.3%）和Streptomyces sp. H14505（98.3%）密切相关，并与Streptomyces sp. H14505形成一个分支。此外，菌株NEAU-YM18的全基因组系统发育显示该菌株形成一个独立的进化枝。NEAU-YM18与Streptomyces sp. 2-25(1)、Streptomyces sp. NEAU-JM1和Streptomyces sp. H14505之间的数字DNA-DNA杂交结果分别为25.0%、24.7%和24.7%，它们之间的全基因组平均核苷酸同一性值分别为81.5%、81.4%和81.4%。这些遗传结果和一些表型特征可以将菌株NEAU-YM18与其参考菌株区分开来。此外，基因组分析证实菌株NEAU-YM18具有分解纤维素和产生生物活性化合物的潜力。因此，菌株NEAU-YM18代表该属的一个新物种，为此提出新种名为Streptomyces langfangensis sp. nov.。模式菌株为NEAU-YM18（=CCTCC AA 2020040=JCM 33977）。
