Department of Food and Animal Sciences, Alabama A & M University, Normal AL 35762, USA.
Department of Food and Animal Sciences, Alabama A & M University, Normal AL 35762, USA.
Poult Sci. 2022 Jul;101(7):101945. doi: 10.1016/j.psj.2022.101945. Epub 2022 May 1.
There is limited information on the effect of exogenous ghrelin infusion on feed intake (FI) in chickens. Therefore, male broilers were used in 3 factorial experiments to determine the relationships between doses (0, 1, or 4 nM; Dose), frequency (once every two h; 2 h), once every 4th h (4 h) or continuous infusion, and ghrelin forms including acylated-ghrelin (AG) and desacylated-ghrelin (DAG) on FI, ADG, and concentrations of corticosterone and Growth Hormone (GH). Treatments were delivered via a jugular cannula, using programmable pumps for 11 consecutive days. FI and ADG were recorded, and plasma was collected. Data were analyzed using a factorial design. In Experiment 1 the effect of AG pulse frequency and doses were evaluated. There was a linear decrease in FI (P = 0.002) and a linear increase in corticosterone (P = 0.033) and GH (P = 0.011) concentrations when AG was infused. However, ADG decreased with doses (P = 0.011) only when AG was given at 2 h. In Experiment 2 the effect of ghrelin forms and doses given at 2 h was evaluated. There was a linear decrease in FI when AG was infused and a linear increase in FI when DAG was infused (P < 0.05). Birds infused with DAG gained more weight than those infused with AG. There was a linear increase in corticosterone and GH concentrations only when AG was infused (P < 0.01). In Experiment 3 the effect of continuous infusion of 2 doses (0 and 1 nM) of AG and DAG were evaluated. There was a linear decrease in FI and ADG when AG (P < 0.001) was infused and a linear increase in FI and ADG when DAG was infused (P < 0.05). There was an increase in corticosterone concentrations only when AG was infused (P = 0.022). However, GH concentrations were not affected by treatments. We concluded that AG and DAG pulse frequency and doses had a differential effect on FI, ADG, corticosterone, and GH concentrations in broiler chickens.
关于外源性生长激素释放肽输注对鸡采食量（FI）的影响，信息有限。因此，本研究在 3 个析因实验中使用雄性肉鸡，以确定剂量（0、1 或 4 nM；剂量）、频率（每 2 小时一次；2 h）、每 4 小时一次（4 h）或连续输注，以及包括酰化生长激素释放肽（AG）和去酰化生长激素释放肽（DAG）在内的生长激素释放肽形式对 FI、平均日增重（ADG）以及皮质酮和生长激素（GH）浓度的关系。通过颈静脉插管和可编程泵连续 11 天给药。记录 FI 和 ADG，并采集血浆。使用析因设计进行数据分析。在实验 1 中，评估了 AG 脉冲频率和剂量的影响。当 AG 输注时，FI 呈线性下降（P = 0.002），皮质酮（P = 0.033）和 GH（P = 0.011）浓度呈线性增加。然而，仅当 AG 在 2 h 时给予时，ADG 才随剂量减少（P = 0.011）。在实验 2 中，评估了 2 h 时生长激素释放肽形式和剂量的影响。当 AG 输注时，FI 呈线性下降，当 DAG 输注时，FI 呈线性增加（P < 0.05）。与输注 AG 的鸟类相比，输注 DAG 的鸟类体重增加更多。仅当 AG 输注时，皮质酮和 GH 浓度呈线性增加（P < 0.01）。在实验 3 中，评估了连续输注 2 种剂量（0 和 1 nM）AG 和 DAG 的效果。当 AG 输注时（P < 0.001），FI 和 ADG 呈线性下降，当 DAG 输注时，FI 和 ADG 呈线性增加（P < 0.05）。仅当 AG 输注时，皮质酮浓度增加（P = 0.022）。然而，GH 浓度不受处理影响。我们得出结论，AG 和 DAG 脉冲频率和剂量对肉鸡的 FI、ADG、皮质酮和 GH 浓度有不同的影响。
