Private Practice, Limassol, Cyprus.
Centre for Oral Clinical Research and Centre for Oral Immunobiology and Regenerative Medicine, Institute of Dentistry, Barts and The London School of Medicine and Dentistry, Queen Mary University of London (QMUL), London, UK.
Clin Oral Investig. 2022 Aug;26(8):5223-5235. doi: 10.1007/s00784-022-04491-0. Epub 2022 Jun 11.
To evaluate the effect of membrane occlusiveness and experimental diabetes on early and late healing following guided bone regeneration.
A total of 30 Wistar rats were randomly allocated to three groups: healthy (H), uncontrolled diabetic (UD) and controlled diabetic (CD). A critical size calvarial defect (CSD) was created at the mid-portion of one parietal bone, and it was treated with a double layer of e-PTFE membrane presenting 0.5 mm perforations. The animals were killed at 7 and 30 days of healing, and qualitative and quantitative histological evaluations were performed. Data were compared with the ones previously obtained from other 30 animals (10H, 10UD, 10 CD), where two CSDs were randomly treated with a double-layer e-PTFE occlusive membrane or left empty.
Following application of cell occlusive or cell permeable membranes, significant regeneration can be observed. However, at 30 days in the H group occlusive compared to cell permeable membranes promoted enhanced bone regeneration (83.9 ± 7.3% vs. 52.5 ± 8.6%), while no significant differences were observed within the CD and UD groups. UD led to reduced regeneration compared to H when an occlusive barrier was applied, whereas comparable outcomes to H and CD were observed when placing perforated membranes.
The application of cell permeable membranes may have masked the potentially adverse effect of experimental UD on bone regeneration.
Membrane porosity might contribute to modulate the bone regenerative response in UD conditions. Future studies are needed to establish the degree of porosity associated with the best regenerative outcomes as well as the underlying molecular mechanisms.
评估膜封闭性和实验性糖尿病对引导骨再生早期和晚期愈合的影响。
将 30 只 Wistar 大鼠随机分为三组：健康组（H）、未控制型糖尿病组（UD）和控制型糖尿病组（CD）。在一块顶骨的中部分别创建一个临界尺寸颅骨缺损（CSD），并用双层 e-PTFE 膜（有 0.5mm 穿孔）进行处理。动物在愈合的第 7 天和第 30 天被处死，并进行定性和定量组织学评估。将数据与之前从另外 30 只动物（10H、10UD、10CD）中获得的数据进行比较，其中两个 CSD 随机用双层 e-PTFE 封闭膜或不处理进行处理。
在应用细胞封闭或细胞通透膜后，可以观察到明显的再生。然而，在 H 组中，与细胞通透膜相比，30 天时封闭膜促进了更强的骨再生（83.9±7.3%对 52.5±8.6%），而在 CD 和 UD 组中则没有观察到显著差异。与 H 组相比，当应用封闭屏障时，UD 导致再生减少，而当放置穿孔膜时，UD 的结果与 H 和 CD 相似。
应用细胞通透膜可能掩盖了实验性 UD 对骨再生的潜在不利影响。
膜的孔隙率可能有助于调节 UD 条件下的骨再生反应。需要进一步的研究来确定与最佳再生结果相关的孔隙率程度以及潜在的分子机制。
