Centre for Clinical Oral Research, Institute of Dentistry, Barts and The London School of Medicine and Dentistry, Queen Mary University of London (QMUL), London, UK.
Periodontology Department, UCL Eastman Dental Institute, London, UK.
Clin Oral Implants Res. 2017 Sep;28(9):e135-e145. doi: 10.1111/clr.12950. Epub 2016 Sep 1.
To identify and describe protein expression in a Wistar rat calvarial critical size defect (CSD) model following treatment with guided bone regeneration in healthy and osteoporotic conditions.
Thirty-six 10-month-old female Wistar rats were used. Half of them were ovariectomized (OVX) and fed with a low-calcium diet to induce an osteoporotic-like status. In each animal of both groups, two 5-mm calvarial CSDs were treated with deproteinized bovine bone mineral graft particles and a bilayer collagen membrane. Six OVX and six control rats were randomly euthanized at 7, 14, and 30 days. One defect/animal was randomly chosen for proteomic analysis. Differently expressed proteins between the two groups were identified with matrix-assisted laser desorption time-of-flight mass spectrometry and liquid chromatography-mass spectrometry/mass spectrometry.
At 7 days, 29 and 27 proteins were, respectively, identified in the healthy and OVX animals. At 14 days, 103 proteins were detected in the healthy controls and 20 proteins in the OVX rats, while at 30 days, 31 and 75 proteins were identified, respectively. Only limited proteins known to play a role in the later stages of bone formation and maturation were identified within the animals 'proteomes.
The osseous formation process was quite immature even at 30 days of healing. An overexpression of inflammatory and stress response pathways was detected in the OVX animals, as well as a tendency toward a delayed maturation of the osseous wound and a reduced/delayed differentiation of osteoblast cell precursors.
在健康和骨质疏松条件下，通过引导骨再生治疗，鉴定并描述 Wistar 大鼠颅骨临界尺寸缺损（CSD）模型中的蛋白质表达。
使用 36 只 10 月龄雌性 Wistar 大鼠。其中一半进行卵巢切除术（OVX）并喂食低钙饮食，以诱导骨质疏松样状态。在两组的每只动物中，两个 5mm 颅骨 CSD 均用脱蛋白牛骨矿物质移植物颗粒和双层胶原膜治疗。每组 6 只 OVX 和 6 只对照大鼠分别在第 7、14 和 30 天随机处死。每只动物随机选择一个缺陷用于蛋白质组学分析。用基质辅助激光解吸飞行时间质谱和液相色谱-质谱/质谱鉴定两组之间差异表达的蛋白质。
在第 7 天，健康组和 OVX 组分别鉴定出 29 种和 27 种蛋白质。在第 14 天，健康对照组检测到 103 种蛋白质，OVX 大鼠检测到 20 种蛋白质，而在第 30 天，分别鉴定出 31 种和 75 种蛋白质。在动物的蛋白质组中仅鉴定出有限的已知在骨形成和成熟后期发挥作用的蛋白质。
即使在 30 天的愈合期，骨形成过程也相当不成熟。在 OVX 动物中检测到炎症和应激反应途径的过度表达，以及骨伤口成熟的延迟趋势和成骨细胞前体分化的减少/延迟。
