来自面包酵母（酿酒酵母）脂质颗粒中的三酰甘油合成。

Triacylglycerol synthesis in lipid particles from baker's yeast (Saccharomyces cerevisiae).

作者信息

Christiansen K

出版信息

Biochim Biophys Acta. 1978 Jul 25;530(1):78-90. doi: 10.1016/0005-2760(78)90128-5.

Abstract

Triacylglycerol synthesis has been studied in a lipid particle preparation of baker's yeast (Saccharomyces cerevisiae), and compared with the synthesis in other subcellular fractions. Fatty acid-CoA ligase (AMP) (EC 6.2.1.3) activity and sn-glycerol 3-phosphate acyltransferase activity (EC 2.3.1.15) were present in all the subcellular fractions tested but the highest specific activities of both enzymes were observed with the lipid particle fraction. The products of the glycerol 3-phosphate acylation indicate that triacyglycerol synthesis proceeds through the phosphatidic acid pathway. However, only a small and nearly constant amount of lysophosphatidic acid was found with the lipid particle fraction while the other subcellular fraction produced lysophosphatidic and phosphatidic acid with a more pronounced precursor/product relationship. Triacylglycerol synthesis from endogenous diacylglycerol present in the lipid particle was also demonstrated.

摘要

已对面包酵母（酿酒酵母）脂质颗粒制剂中的三酰甘油合成进行了研究，并与其他亚细胞组分中的合成进行了比较。在所测试的所有亚细胞组分中均存在脂肪酸 - 辅酶A连接酶（AMP）（EC 6.2.1.3）活性和sn - 甘油3 - 磷酸酰基转移酶活性（EC 2.3.1.15），但在脂质颗粒组分中观察到这两种酶的比活性最高。甘油3 - 磷酸酰化产物表明三酰甘油合成通过磷脂酸途径进行。然而，在脂质颗粒组分中仅发现少量且几乎恒定的溶血磷脂酸，而其他亚细胞组分产生溶血磷脂酸和磷脂酸，且前体/产物关系更为明显。还证实了脂质颗粒中存在的内源性二酰甘油合成三酰甘油。