Ferreira Letícia Rocha, de Almeida Thaiza Teixeira, Andretta Milimani, Perin Luana Martins, Camargo Anderson Carlos, de Carvalho Antônio Fernandes, Nero Luís Augusto
Departamento de Veterinária, InsPOA - Laboratório de Inspeção de Produtos de Origem Animal, Universidade Federal de Viçosa, Campus Universitário, Centro, Viçosa MG, 36570 900, Brazil.
Departamento de Tecnologia de Alimentos, Inovaleite - Laboratório de Ciência E Tecnologia Do Leite E Derivados, Universidade Federal de Viçosa, Campus Universitário, Centro, Viçosa MG, 36570 900, Brazil.
Braz J Microbiol. 2022 Sep;53(3):1593-1598. doi: 10.1007/s42770-022-00778-2. Epub 2022 Jun 10.
This study aimed to provide a further characterization of the lactic microbiota present in Minas artisanal cheese (MAC) from the Serro region by using culture-independent methods, as a complementary analysis of a previous study. The total DNA extracted from MAC samples (n = 55) was subjected to repetitive extragenic palindromic-PCR (rep-PCR) and PCR-denaturing gradient gel electrophoresis (PCR-DGGE). Rep-PCR analysis showed that core microbiota of Serro MAC was closely related, independent of the production town, farm size, or time of production. The sequencing of PCR-DGGE bands identified the prevalence of Lactococcus lactis in all samples, and Streptococcus salivarius was also identified. Thus, we conclude that when more accurate methods are unavailable, rep-PCR can be used as a culture-independent method to demonstrate if the microbiota is closely related or not among the samples. PCR-DGGE results also matched to the main findings of high-throughput sequencing, previously presented, confirming its confidence to detect the main microbial groups present in the raw milk cheeses.
本研究旨在通过使用非培养方法，对来自塞罗地区的手工制作的米纳斯奶酪（MAC）中存在的乳酸微生物群进行进一步表征，作为先前一项研究的补充分析。从MAC样品（n = 55）中提取的总DNA进行了重复外显子回文PCR（rep-PCR）和PCR-变性梯度凝胶电泳（PCR-DGGE）。Rep-PCR分析表明，塞罗MAC的核心微生物群密切相关，与生产城镇、农场规模或生产时间无关。PCR-DGGE条带的测序确定了所有样品中乳酸乳球菌的普遍性，还鉴定出了唾液链球菌。因此，我们得出结论，当没有更准确的方法时，rep-PCR可以用作一种非培养方法来证明样品之间的微生物群是否密切相关。PCR-DGGE结果也与先前呈现的高通量测序的主要结果相匹配，证实了其检测生乳奶酪中主要微生物群的可信度。
