White R H, Rudolph F B
Biochim Biophys Acta. 1978 Aug 17;542(2):340-7. doi: 10.1016/0304-4165(78)90029-6.
Methods are described for the isolation and gas chromatographic-mass spectrometric analysis of the 4-methyl-5-beta-hydroxyethyl thiazole moiety of thiamine in microbial cells. Using these methods, it was determined that in Escherichia coli the nitrogen atom in the thiazole ring of thiamine is derived solely from L-tyrosine.
描述了从微生物细胞中分离硫胺素的4-甲基-5-β-羟乙基噻唑部分并进行气相色谱-质谱分析的方法。使用这些方法确定,在大肠杆菌中,硫胺素噻唑环中的氮原子仅来源于L-酪氨酸。