鼠伤寒沙门氏菌中硫胺素噻唑部分的生物合成。

The biosynthesis of the thiazole moiety of thiamine in Salmonella Typhimurium.

作者信息

Bellion E, Kirkley D H, Faust J R

出版信息

Biochim Biophys Acta. 1976 Jun 23;437(1):229-37. doi: 10.1016/0304-4165(76)90364-0.

Abstract

The mechanism of biosynthesis of 4-methyl-5-beta-hydroxyethyl thiazole, the thiazole moiety of thiamine was studied in Salmonella typhimurium. Using the adenosine derepression technique the incorporation of various 14C-labeled precursors was determined. We found that;e1Me-14C]methionine, [2-14C]methionine, [U-14C]alanine, and [2-14C]glycine were not incorporated whereas [2-14C]tyrosine was incorporated. Degradation of the 4-methyl-5-beta-hydroxyethyl thiazole obtained after [2-14C]tyrosine incorporation revealed that all of the activity was located on carbon-2. These findings are discussed and compared with previous findings concerning 4-methyl-5-beta-hydroxyethyl thiazole biosynthesis.

摘要

在鼠伤寒沙门氏菌中研究了硫胺素的噻唑部分4-甲基-5-β-羟乙基噻唑的生物合成机制。采用腺苷去阻遏技术测定了各种14C标记前体的掺入情况。我们发现，[e1Me-14C]甲硫氨酸、[2-14C]甲硫氨酸、[U-14C]丙氨酸和[2-14C]甘氨酸未被掺入，而[2-14C]酪氨酸被掺入。[2-14C]酪氨酸掺入后得到的4-甲基-5-β-羟乙基噻唑的降解表明，所有活性都位于碳-2上。对这些发现进行了讨论，并与先前关于4-甲基-5-β-羟乙基噻唑生物合成的发现进行了比较。