School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, P. R. China.
National Institutes for Food and Drug Control, Beijing, 102629, P. R. China.
Chem Biodivers. 2022 Jul;19(7):e202200218. doi: 10.1002/cbdv.202200218. Epub 2022 Jun 27.
Three new alkamides, achilleamide B-D (1-3) along with five known alkamides (4-8) were isolated from the aerial parts of Achillea alpina L. Structures were elucidated by spectroscopic analysis. Modified Mosher's method and electronic circular dichroism (ECD) calculations were introduced for the absolute configuration of 3. The neuroprotective effects of all the compounds were evaluated by 6-hydroxydopamine (6-OHDA)-induced cell death in human neuroblastoma SH-SY5Y cells, with concentration for 50 % of maximal effect (EC ) values of 3.16-24.75 μM, and the structure-activity relationship was conducted.
从高山蓍（Achillea alpina L.）的地上部分分离得到了三种新的酰胺类化合物，即 Achilleamide B-D（1-3），以及五种已知的酰胺类化合物（4-8）。通过光谱分析阐明了它们的结构。采用改进的 Mosher 法和电子圆二色谱（ECD）计算确定了 3 的绝对构型。通过 6-羟多巴胺（6-OHDA）诱导的人神经母细胞瘤 SH-SY5Y 细胞死亡评估了所有化合物的神经保护作用，化合物的半数最大效应浓度（EC ）值为 3.16-24.75 μM，同时进行了构效关系研究。
