Walts A E, Said J W, Shintaku I P
Diagn Cytopathol. 1987 Mar;3(1):41-9. doi: 10.1002/dc.2840030109.
Immunoreactivity for epithelial membrane antigen (EMA) was evaluated in exfoliated benign and malignant cells using immunoperoxidase and immunogold techniques. In addition, protein A-colloidal gold was used for ultrastructural localization of EMA. Our results suggest that EMA is useful in distinguishing adenocarcinoma cells (strongly positive) from reactive mesothelial cells (negative or weakly positive), lymphoid cells (negative), and a variety of nonepithelial neoplasms (negative) with which they may be confused. Exfoliated cells from two mesotheliomas were also strongly positive for EMA. Ultrastructurally, EMA was distributed in a dense, even, linear pattern along the cell membrane and microvillous surface processes of adenocarcinoma cells. A similar but sparse distribution pattern was observed in reactive mesothelial cells. These observations reflect the increased sensitivity and higher resolution of the immunogold technique.
采用免疫过氧化物酶和免疫金技术，对脱落的良性和恶性细胞中的上皮膜抗原（EMA）免疫反应性进行了评估。此外，蛋白A-胶体金用于EMA的超微结构定位。我们的结果表明，EMA有助于将腺癌细胞（强阳性）与反应性间皮细胞（阴性或弱阳性）、淋巴细胞（阴性）以及可能与之混淆的各种非上皮性肿瘤（阴性）区分开来。来自两例间皮瘤的脱落细胞对EMA也呈强阳性。在超微结构上，EMA沿腺癌细胞的细胞膜和微绒毛表面突起呈致密、均匀的线性分布。在反应性间皮细胞中观察到类似但稀疏的分布模式。这些观察结果反映了免疫金技术更高的敏感性和分辨率。
