van der Kwast T H, Versnel M A, Delahaye M, de Jong A, Zondervan P E, Hoogsteden H
Department of Pathology, Academic Hospital Dijkzigt, Rotterdam, The Netherlands.
Acta Cytol. 1988 Mar-Apr;32(2):169-74.
The presence of epithelial membrane antigen (EMA) on malignant mesothelial cells found in pleural and ascitic fluids was demonstrated immunocytochemically using a monoclonal anti-EMA antibody. Serous fluids of 25 patients with malignant mesotheliomas were investigated. In 23 cases, varying numbers of EMA-positive tumor cells were present; in 2 cases, no such cells were found. Immunoelectron microscopy was performed both on Lowicryl-embedded sediments of serous fluids and by application of preembedding techniques using the immunogold method. Expression of EMA by the immunogold method was found selectively on the villi of the malignant mesothelioma cells whereas the nonvillous, flat surfaces were largely EMA-negative. The results indicate that immunoelectron microscopy may offer a useful adjunct in the diagnosis of malignant mesothelioma in serous fluids.
使用单克隆抗上皮膜抗原（EMA）抗体，通过免疫细胞化学方法证实了在胸腔积液和腹水的恶性间皮细胞中存在EMA。对25例恶性间皮瘤患者的浆液进行了研究。23例中存在数量不等的EMA阳性肿瘤细胞；2例未发现此类细胞。对浆液的Lowicryl包埋沉淀物以及采用免疫金法的包埋前技术进行了免疫电子显微镜检查。通过免疫金法发现EMA选择性地表达于恶性间皮瘤细胞的绒毛上，而非绒毛状的平坦表面大多为EMA阴性。结果表明，免疫电子显微镜检查可能为浆液中恶性间皮瘤的诊断提供有用的辅助手段。
