Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Mushtuhur, Touch, Al Qalyubia Governorate, Benha, Egypt.
Biol Trace Elem Res. 2023 Apr;201(4):1935-1945. doi: 10.1007/s12011-022-03298-0. Epub 2022 Jun 11.
Prostate cancer (PCa) is the most diagnosed cancer in 112 countries and the second leading cause of death in men in 48 countries. We studied the outstanding agents silver nanoparticles (AgNPs) and Spirulina algae (Sp) for the management of PCa once as monotherapy or last as a combination. PCa in rats was induced using bicalutamide (Casodex®) and testosterone, followed by (7, 12-dimethylbenz[a]anthracene). Then, testosterone was injected s.c. for 3 months. Rats were divided into six groups, with 12 rats in each group. Group I was assigned as the control (co), group II as the PCa model, group III treated with AgNPs, group IV treated with Spirulina extract, group V treated with a combination of AgNPs plus Spirulina, and group VI treated with bicalutamide. The results show that AgNPs could normalize IL-6 levels and could overcome the hormonal disturbance induced in PCa rats along the hypothalamic-pituitary-testis axis. Spirulina revealed a significant reduction in the level of total and free prostatic specific antigen (PSA) to the same level as bicalutamide treatment, which was the same as the control group. Histopathological study revealed regression (75%) of the histological pattern of high-grade prostatic intraepithelial neoplasia (HGPIN) for Spirulina alone, and (50%) for bicalutamide. The best effect on IL-6 decline was reached with the AgNPs/Spirulina combination as well as bicalutamide treatment compared with the PCa group. Bicalutamide treatment significantly decreased the PSA concentration relative to the PCa group and reached the normal level. Adding Spirulina to AgNPs as a combination enhanced its effect on all mentioned drawbacks associated with PCa except hormonal imbalance that needs more adjustments.
前列腺癌（PCa）是 112 个国家中诊断率最高的癌症，也是 48 个国家中男性的第二大死亡原因。我们研究了卓越的药剂——银纳米粒子（AgNPs）和螺旋藻（Sp），用于 PCa 的管理，既可以作为单一疗法，也可以作为联合疗法。使用比卡鲁胺（Casodex®）和睾丸酮诱导大鼠 PCa，然后用（7,12-二甲基苯并[a]蒽）处理。然后，睾丸酮通过皮下注射 3 个月。将大鼠分为 6 组，每组 12 只。第 I 组被分配为对照组（co），第 II 组为 PCa 模型组，第 III 组用 AgNPs 处理，第 IV 组用螺旋藻提取物处理，第 V 组用 AgNPs 和螺旋藻的混合物处理，第 VI 组用比卡鲁胺处理。结果表明，AgNPs 可以使 IL-6 水平正常化，并可以克服 PCa 大鼠沿下丘脑-垂体-睾丸轴引起的激素紊乱。螺旋藻显著降低总前列腺特异抗原（PSA）和游离 PSA 的水平，与比卡鲁胺治疗水平相同，与对照组相同。组织病理学研究显示，螺旋藻单独治疗可使高级别前列腺上皮内瘤变（HGPIN）的组织学模式消退（75%），比卡鲁胺治疗可使（50%）消退。与 PCa 组相比，AgNPs/螺旋藻联合治疗和比卡鲁胺治疗对 IL-6 下降的效果最好。与 PCa 组相比，比卡鲁胺治疗显著降低了 PSA 浓度，达到了正常水平。将螺旋藻添加到 AgNPs 作为联合治疗增强了其对与 PCa 相关的所有缺陷的作用，除了激素失衡，这需要更多的调整。
