Quintar Amado A, Gonçalves Bianca F, Taboga Sebastião R, Maldonado Cristina A
Centro de Microscopía Electrónica, Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas (IBILCE), Universidade. Estadual Paulista (UNESP), São José do Rio Preto, Brazil.
Cell Biol Int. 2017 Nov;41(11):1234-1238. doi: 10.1002/cbin.10789. Epub 2017 May 25.
One of the recognized issues in prostate cancer research is the lack of animal models allowing the research of pathological, biochemical, and genetic factors in immunocompetent animals. Our research group has successfully employed the gerbil in several studies for prostate diseases. In the present work, we aimed to analyze the effect of chronic bacterial inflammation on N-methyl-N-nitrosourea (MNU)-induced prostate carcinogenesis in gerbils. Histopathological assessment of the prostatic complex revealed that treatment combinations with MNU plus testosterone or bacterial infection resulted in a promotion of prostate cancer, with bacterial inflammation being more effective in increasing premalignant and malignant tissular alterations than testosterone in the prostate. Furthermore, chronic bacterial inflammation itself induced premalignant lesions in the ventral lobe and increased their frequency in the dorsolateral lobe as well as malignant lesions in the ventral prostate. These animals showed a rich inflammatory microenvironment, characterized as intraluminal and periductal foci. These data indicate that chronic inflammation induced by Escherichia coli acts as a potent tumor promoter, in the early stages of carcinogenesis in the gerbil, in line with the hypothesis of inflammation supporting several steps of tumor development in the prostate gland.
前列腺癌研究中公认的问题之一是缺乏能在具有免疫活性的动物中研究病理、生化和遗传因素的动物模型。我们的研究小组已在多项前列腺疾病研究中成功使用沙鼠。在本研究中，我们旨在分析慢性细菌炎症对N-甲基-N-亚硝基脲（MNU）诱导的沙鼠前列腺癌发生的影响。前列腺复合体的组织病理学评估显示，MNU联合睾酮或细菌感染的治疗组合会促进前列腺癌的发生，与睾酮相比，细菌炎症在增加前列腺癌前和恶性组织改变方面更有效。此外，慢性细菌炎症本身会在腹叶诱导癌前病变，并增加背外侧叶的癌前病变频率以及腹侧前列腺的恶性病变。这些动物表现出丰富的炎症微环境，其特征为管腔内和导管周围病灶。这些数据表明，在沙鼠致癌作用的早期阶段，大肠杆菌诱导的慢性炎症是一种有效的肿瘤促进剂，这与炎症支持前列腺肿瘤发展多个步骤的假说一致。
