Lee S S, Mohabbat M O, Burzynski S R
Drugs Exp Clin Res. 1987;13 Suppl 1:13-6.
Antineoplaston A3 is an oxidized mixture of small and medium size peptides and amino acid derivatives isolated from human urine. The purpose of this study was to evaluate the growth inhibitory effect of Antineoplaston A3 on cancer cells in vitro and its toxicity in mice. The growth inhibitory studies were carried out on breast carcinoma cells HBL-100. The cells were plated at 1000 cells per flask in L-15 medium and supplemented with 10% foetal bovine serum and antibiotics. Two different experiments were performed: to study dose response; and to investigate duration exposure. To establish dose response, Antineoplaston A3 was added to each flask to a final concentration of 0.05, 0.1, 0.2 and 0.4 mg/ml. Two flasks in each test were added with the same amount of medium to serve as control. The flasks were incubated at 37 degrees C for 6 days, then fixed and stained, after which colonies were counted. Results were expressed as percentage of control. In duration exposure experiments Antineoplaston A3 was added to the final concentration of 0.4 mg/ml. After 24, 48 and 72 h of exposure to Antineoplaston A3, the flasks were returned to normal growth medium and colonies were again counted. Dose response to Antineoplaston A3 was as follows (average of 3 replications +/- standard error): 0.05 mg/ml, 100% +/- 0; 0.1 mg/ml, 79.0% +/- 14.4; 0.2 mg/ml, 4.3% +/- 6.6; 0.4 mg/ml, 0% +/- 0.(ABSTRACT TRUNCATED AT 250 WORDS)
抗瘤酮A3是从人尿中分离出的中小分子肽和氨基酸衍生物的氧化混合物。本研究的目的是评估抗瘤酮A3对体外癌细胞的生长抑制作用及其对小鼠的毒性。对乳腺癌细胞HBL - 100进行了生长抑制研究。将细胞以每瓶1000个细胞接种于L - 15培养基中，并添加10%胎牛血清和抗生素。进行了两项不同的实验：研究剂量反应；以及研究持续暴露时间。为确定剂量反应，向每个培养瓶中加入抗瘤酮A3，使其终浓度分别为0.05、0.1、0.2和0.4mg/ml。每个试验中两个培养瓶加入等量培养基作为对照。将培养瓶在37℃孵育6天，然后固定染色，之后计数集落。结果以对照的百分比表示。在持续暴露实验中，抗瘤酮A3的终浓度为0.4mg/ml。在暴露于抗瘤酮A3 24、48和72小时后，将培养瓶放回正常生长培养基中，再次计数集落。抗瘤酮A3的剂量反应如下（3次重复的平均值±标准误差）：0.05mg/ml，100%±0；0.1mg/ml，79.0%±14.4；0.2mg/ml，4.3%±6.6；0.4mg/ml，0%±0。（摘要截短于250字）
