CSIR-National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR-NBRI), Rana Pratap Marg, Lucknow 226001, India.
CSIR-National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR-NBRI), Rana Pratap Marg, Lucknow 226001, India; Academy of Scientific and Innovative Research (AcSIR), CSIR- Human Resource Development Centre, (CSIR-HRDC) Campus, Postal Staff College Area, Sector 19, Kamla Nehru Nagar, Ghaziabad, Uttar Pradesh 201 002, India.
Gene. 2022 Aug 20;836:146666. doi: 10.1016/j.gene.2022.146666. Epub 2022 Jun 8.
MADS box gene family is transcription factor gene family that is involved in growth and development of eukaryotes. In plants the MADS box gene family is mainly associated with floral meristem identity and flower development, apart from being involved in nearly all the phases of plant growth. The MADS box gene family has also been shown to be involved during fruit development and ripening. In this study the MADS box gene family from Musa balbisiana was identified and the divergence of this gene family between Musa balbisiana and Musa acuminata studied. A total of 97 MADS box genes were identified from the genome of Musa balbisiana. Phylogenetic analysis showed that the MbMADS box genes were categorised into type I (α and γ; the β group was not distinguishable) and type II groups (MIKC and MIKC* and MIKC was further divided into 13 subfamilies). The typeII group has the largest number of genes and also showed the most expansion which could be correlated with the whole genome duplications. There were significant differences in the MADS box genes from Musa acuminata and Musa balbisiana during evolution that can be correlated with different floral phenotype and fruit ripening pattern. The divergence of the MADS RIN genes in Musa balbisiana as compared to Musa acuminata might play an important role in the slow ripening of Musa balbisiana fruits.
MADS 盒基因家族是参与真核生物生长和发育的转录因子基因家族。在植物中，MADS 盒基因家族主要与花分生组织身份和花发育有关，除了参与植物生长的几乎所有阶段外。MADS 盒基因家族也被证明在果实发育和成熟过程中发挥作用。在这项研究中，鉴定了 Musa balbisiana 中的 MADS 盒基因家族，并研究了该基因家族在 Musa balbisiana 和 Musa acuminata 之间的分化。从 Musa balbisiana 的基因组中鉴定出了 97 个 MADS 盒基因。系统发育分析表明，MbMADS 盒基因分为 I 型（α和γ；β 组不可区分）和 II 型（MIKC 和 MIKC*，MIKC 进一步分为 13 个亚家族）。II 型组具有最多的基因，并且显示出最大的扩张，这可能与全基因组加倍有关。在进化过程中，Musa acuminata 和 Musa balbisiana 中的 MADS 盒基因存在显著差异，这与不同的花表型和果实成熟模式有关。与 Musa acuminata 相比，Musa balbisiana 中 MADS RIN 基因的分化可能在 Musa balbisiana 果实的缓慢成熟中发挥重要作用。
