College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, 150030, China.
College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, 150030, China; Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin, Heilongjiang, 150030, China.
Food Microbiol. 2022 Sep;106:104059. doi: 10.1016/j.fm.2022.104059. Epub 2022 May 16.
Thirty-seven lactic acid bacteria (LAB) strains were isolated from traditional dry sausages collected from Northeast China, including Latilactobacillus sakei (29 strains), Lactiplantibacillus plantarum (4 strains), Latilactobacillus curvatus (2 strains), Weissella hellenica (1 strain), and Lactococcus lactis (1 strain). Some LAB strains had tolerance to high concentrations of sodium chloride (6%), sodium nitrite (150 mg/L NaNO), and acid (pH 4.0). They showed good growth and acidification properties and antimicrobial activity. Among them, five LAB strains that exhibited the best technological properties were selected and inoculated in the sausage model to explore their roles in flavor development. The contents of total free amino acids (FAAs) decreased ranging from 109.11 mg/g to 58.06 mg/g. A total of 46 volatile compounds were identified and the contents of volatile compounds increased in the sausage model during fermentation. Partial least squares regression analysis showed that Lb. sakei HRB10, Lb. plantarum MDJ2, W. hellenica HRB6, and Lc. lactis HRB0 promoted the generation of FAAs and volatile compounds in the sausage model. These findings demonstrated that the autochthonous LAB species are promising for the production of sausage with better flavor and fermentation performance.
从中国东北地区收集的传统干香肠中分离出 37 株乳酸菌(LAB)菌株,包括清酒乳杆菌(29 株)、植物乳杆菌(4 株)、弯曲乳杆菌(2 株)、希氏乳杆菌(1 株)和乳酸乳球菌(1 株)。一些 LAB 菌株具有耐受高浓度氯化钠(6%)、亚硝酸钠(150mg/L NaNO)和酸(pH 4.0)的能力。它们表现出良好的生长和酸化特性以及抗菌活性。其中,选择了 5 株表现出最佳技术特性的 LAB 菌株接种到香肠模型中,以探索它们在风味发展中的作用。总游离氨基酸(FAAs)含量从 109.11mg/g 降至 58.06mg/g。鉴定出 46 种挥发性化合物,在发酵过程中香肠模型中的挥发性化合物含量增加。偏最小二乘回归分析表明,Lb. sakei HRB10、Lb. plantarum MDJ2、W. hellenica HRB6 和 Lc. lactis HRB0 促进了香肠模型中 FAAs 和挥发性化合物的生成。这些发现表明,土著 LAB 物种有望生产出具有更好风味和发酵性能的香肠。