Li Nan, Jiang Dongya, He Luqingqing, Yue Yunyun, Zhang Qinxin, Wang Shuang, Zhang Yunfeng, Wei Yuxuan, Zhao Qingshun
Model Animal Research Center, Medical School, Nanjing University, NanjingJiangsu, 210061, China.
School of Traditional Chinese Pharmacy, China Pharmaceutical University, NanjingJiangsu, 210009, China.
Cell Biosci. 2022 Jun 11;12(1):87. doi: 10.1186/s13578-022-00825-3.
In the evolutionary "arms race" from prokaryotes to eukaryotes, some memories of foreign DNA have been conserved for defensive purposes. Shortly after invasion by the plasmid, pEGFP-N1, the conserved the defense gene, isg15, was activated in the zebrafish zygote and in mammalian cells. Based on the sequence similarity, we found three virus-derived sequences in pEGFP-N1 which share the 5'-GTTTGTT-3' core sequence, an epigenetic factor leading to increased expression of isg15. Mutation of the core sequence greatly reduces the degradation rate of the plasmid in E. coli cells or zebrafish embryos. We conclude that a conserved defense response, common to both eukaryotic and prokaryotic cells, allows identification and degradation of plasmids containing 5'-GTTTGTT-3'.
在从原核生物到真核生物的进化“军备竞赛”中，一些对外源DNA的记忆被保留下来用于防御。质粒pEGFP-N1侵入后不久，保守的防御基因isg15在斑马鱼受精卵和哺乳动物细胞中被激活。基于序列相似性，我们在pEGFP-N1中发现了三个病毒衍生序列，它们共享5'-GTTTGTT-3'核心序列，这是一个导致isg15表达增加的表观遗传因子。核心序列的突变大大降低了质粒在大肠杆菌细胞或斑马鱼胚胎中的降解率。我们得出结论，真核细胞和原核细胞共有的一种保守防御反应能够识别并降解含有5'-GTTTGTT-3'的质粒。
