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[牛胎儿胰岛细胞单层培养物的胰岛素生成活性]

[Insulin-forming activity of monolayer cultures of pancreatic islet cells from cattle fetuses].

作者信息

Fedotov V P, Bliumkin V N, Babikova R A, Sadovnikova N V, Abramova V V

出版信息

Biull Eksp Biol Med. 1978 Aug;86(8):235-8.

PMID:356913
Abstract

The radioimmunological method was applied to the study of insulin content in the growth medium of primary monolayer cultures of bovine fetal pancreatic islet cells grown with usual and increased (300 mg%) glucose content. The latter led to an enhanced insulin secretion. The results of cytological study demonstrated a definite interrelationship between the mitotic activity of culture cells and the intensity of insulin secretion into the medium.

摘要

采用放射免疫法研究了在葡萄糖含量正常和增加(300mg%)的情况下,牛胎儿胰岛细胞原代单层培养生长培养基中的胰岛素含量。后者导致胰岛素分泌增加。细胞学研究结果表明,培养细胞的有丝分裂活性与培养基中胰岛素分泌强度之间存在一定的相互关系。

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