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[牛胎儿胰岛细胞单层培养物的胰岛素生成活性]

[Insulin-forming activity of monolayer cultures of pancreatic islet cells from cattle fetuses].

作者信息

Fedotov V P, Bliumkin V N, Babikova R A, Sadovnikova N V, Abramova V V

出版信息

Biull Eksp Biol Med. 1978 Aug;86(8):235-8.

PMID:356913
Abstract

The radioimmunological method was applied to the study of insulin content in the growth medium of primary monolayer cultures of bovine fetal pancreatic islet cells grown with usual and increased (300 mg%) glucose content. The latter led to an enhanced insulin secretion. The results of cytological study demonstrated a definite interrelationship between the mitotic activity of culture cells and the intensity of insulin secretion into the medium.

摘要

采用放射免疫法研究了在葡萄糖含量正常和增加(300mg%)的情况下,牛胎儿胰岛细胞原代单层培养生长培养基中的胰岛素含量。后者导致胰岛素分泌增加。细胞学研究结果表明,培养细胞的有丝分裂活性与培养基中胰岛素分泌强度之间存在一定的相互关系。

相似文献

1
[Insulin-forming activity of monolayer cultures of pancreatic islet cells from cattle fetuses].[牛胎儿胰岛细胞单层培养物的胰岛素生成活性]
Biull Eksp Biol Med. 1978 Aug;86(8):235-8.
2
In vitro regulation of insulin release and biosynthesis of fetal rat pancreatic cells explanted on pregnancy day 16.妊娠第16天取出的胎鼠胰腺细胞胰岛素释放和生物合成的体外调节
Biol Neonate. 1997;71(1):60-8. doi: 10.1159/000244398.
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[Cytological and insulin-producing characteristics of pancreatic islet cell cultures from human fetuses of different ages].[不同年龄人类胎儿胰岛细胞培养物的细胞学及胰岛素分泌特性]
Probl Endokrinol (Mosk). 1985 Nov-Dec;31(6):48-51.
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[Cytological and insulin-producing characteristics of cultures of pancreatic islet cells from newborn piglets].[新生仔猪胰岛细胞培养物的细胞学及胰岛素分泌特性]
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Monolayer culture of neonatal pig pancreatic islet cells.
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Islet cell culture in defined serum-free medium.在限定的无血清培养基中进行胰岛细胞培养。
Endocrinology. 1990 Apr;126(4):1895-903. doi: 10.1210/endo-126-4-1895.
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[Monolayer culture of pancreatic beta cells of newborn rats: Insulin secretion in vitro and attempt at beta-cell transplantation in experimental diabetes].[新生大鼠胰腺β细胞单层培养:体外胰岛素分泌及实验性糖尿病中β细胞移植的尝试]
Probl Endokrinol (Mosk). 1978 Jul-Aug;24(4):57-60.
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[Comparative morphological and insulin-producing characteristics of cellular cultures of the islands of Langerhans in experimental animals].[实验动物胰岛细胞培养物的比较形态学及胰岛素分泌特性]
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Insulin release in response to glucose from isolated mouse, rat, porcine, bovine, and human pancreatic islets.分离的小鼠、大鼠、猪、牛和人类胰岛对葡萄糖作出反应而释放胰岛素。
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Role of polyamines in mitogenic and secretory responses of pancreatic beta-cells to growth factors.多胺在胰腺β细胞对生长因子的促有丝分裂和分泌反应中的作用。
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