Komissarenko V P, Turchin I S, Lazareva N P, Tishchenko A V, Onishchenko D S
Probl Endokrinol (Mosk). 1985 Mar-Apr;31(2):65-8.
The paper is concerned with the characteristics of morphological changes and insulin producing activity of neonatal pig islet cell initial cultures in health and in different glucose concentrations. The investigated cultures preserve morphological and functional differentiation within 4-5 weeks of growth. Insulin biosynthesis and secretion are raised by glucose injection at a dose of 300 mg%. An increase in the dose up to 600 mg% results in the suppression of insulocyte functional activity. The obtained cultures can be used for the treatment of diabetes mellitus.
本文关注新生猪胰岛细胞原代培养物在健康状态以及不同葡萄糖浓度下的形态变化特征和胰岛素分泌活性。所研究的培养物在生长4 - 5周内保持形态和功能分化。注射300mg%的葡萄糖可提高胰岛素的生物合成和分泌。剂量增加至600mg%会导致胰岛细胞功能活性受到抑制。所获得的培养物可用于治疗糖尿病。
