Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet 3114, Bangladesh.
Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet 3114, Bangladesh; Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada.
Gene. 2022 Aug 15;835:146664. doi: 10.1016/j.gene.2022.146664. Epub 2022 Jun 9.
The heavy-metal-associated (HMA) family plays a major role in the transportation of metals. Despite having the genome sequence of the tomato (Solanum lycopersicum), the HMA gene family has not been studied yet. In this study, we identified 48 HMA genes and categorized them into Cu/Ag P1B-ATPase and Zn/Co/Cd/Pb P1BATPase sub-families according to their phylogenic relationship with Arabidopsis and rice. The SlHMA genes were distributed throughout the 12 chromosomes. Analysis of gene structure, chromosomal position, and synteny, revealed that segmental duplications bestowed their evolution. The high numbers of stress-related cis-elements were found to be present in the putative promoter regions indicate the involvement of SlHMAs in stress modulation pathways. RNA-seq data revealed that SlHMAs had divergent expression in different tissues and developmental stages, where members of Cu/Ag P1B-ATPase subfamily were strongly expressed in the roots. RT-qPCR analysis of nine selected SlHMAs showed that most of the genes were up-regulated in response to heavy metals and moderately regulated in response to different abiotic stresses such as salt, drought, and cold.
重金属相关（HMA）家族在金属运输中起着重要作用。尽管番茄（Solanum lycopersicum）具有基因组序列，但尚未对 HMA 基因家族进行研究。在这项研究中，我们鉴定了 48 个 HMA 基因，并根据与拟南芥和水稻的系统发育关系将其分为 Cu/Ag P1B-ATPase 和 Zn/Co/Cd/Pb P1BATPase 亚家族。SlHMA 基因分布在 12 条染色体上。基因结构、染色体位置和共线性分析表明，片段重复赋予了它们的进化。在假定的启动子区域中发现存在大量与应激相关的顺式元件，表明 SlHMAs 参与了应激调节途径。RNA-seq 数据显示，SlHMAs 在不同组织和发育阶段的表达存在差异，Cu/Ag P1B-ATPase 亚家族的成员在根中强烈表达。对 9 个选定的 SlHMAs 的 RT-qPCR 分析表明，大多数基因在重金属胁迫下上调，在盐、干旱和寒冷等不同非生物胁迫下中度调节。
